Rabbit anti-Human, Mouse EPHA2/EPHA3/EPHA4 Polyclonal Antibody | anti-EPHA2 antibody

EPHA2/EPHA3/EPHA4 (Ab-588/596) Antibody

Cat. Num. AAA7132734

• Gene Names: EPHA2; ECK; CTPA; ARCC2; CTPP1; CTRCT6
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunofluorescence
• Purity: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Synonyms: EPHA2/EPHA3/EPHA4; Polyclonal Antibody; EPHA2/EPHA3/EPHA4 (Ab-588/596) Antibody; EC 2.7.10.1; ECK; Ephrin type-A receptor 2 precursor; Epithelial cell kinase; MPK-5; anti-EPHA2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Form/Format: Liquid; Rabbit IgG In Phosphate Buffered Saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% Sodium Azide and 50% Glycerol.

• Applicable Applications for anti-EPHA2 antibody: ELISA (EIA), Western Blot (WB), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:3000; IF: 1:100-1:500
• Immunogen: Synthesized non-phosphopeptide derived from human EPHA2/3/4 around the phosphorylation site of tyrosine 588/596 (T-Y(p)-V-D-P).
• Immunogen Species: Human
• Conjugate: Non-conjugated
• Preparation and Storage: Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.

Immunofluorescence (IF)

(Immunofluorescence analysis of A549 cells, using EPHA2/3/4 (Ab-588/596) antibody.)

Western Blot (WB)

(Western blot analysis of extracts from MCF7 cells, using EPHA2/3/4 (Ab-588/596) antibody.)

Western Blot (WB)

(Western blot analysis of extracts from A549 cells (Lane 2) and HepG2 cells (Lane 3), using EPHA2/3/4 (Ab-588/596) antiobdy. The lane on the left is treated with synthesized peptide.)

Product Categories/Family for anti-EPHA2 antibody

Cardiovascular

NCBI and Uniprot Product Information

• NCBI GI #: 32967311
• NCBI GeneID: 1969
• NCBI Accession #: NP_004422.2
• NCBI GenBank Nucleotide #: NM_004431.3
• UniProt Accession #: P29317; P29320; P54764
• Molecular Weight: 108,266 Da
• NCBI Official Full Name: ephrin type-A receptor 2
• NCBI Official Synonym Full Names: EPH receptor A2
• NCBI Official Symbol: EPHA2
• NCBI Official Synonym Symbols: ECK; CTPA; ARCC2; CTPP1; CTRCT6
• NCBI Protein Information: ephrin type-A receptor 2; soluble EPHA2 variant 1; tyrosine-protein kinase receptor ECK; epithelial cell receptor protein tyrosine kinase
• UniProt Protein Name: Ephrin type-A receptor 2
• Protein Family: Ephrin type-A receptor
• UniProt Gene Name: EPHA2
• UniProt Synonym Gene Names: ECK
• UniProt Entry Name: EPHA2_HUMAN

NCBI Description

This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. This gene encodes a protein that binds ephrin-A ligands. Mutations in this gene are the cause of certain genetically-related cataract disorders.[provided by RefSeq, May 2010]

Uniprot Description

EphA2: a receptor tyrosine kinase. Receptor for members of the ephrin-A family. Binds to ephrin-A1, -A3, -A4 AND -A5. The Eph receptor tyrosine kinase family, the largest in the tyrosine kinase group, has fourteen members. They bind membrane-anchored ligands, ephrins, at sites of cell-cell contact, regulating the repulsion and adhesion of cells that underlie the establishment, maintenance, and remodeling of patterns of cellular organization. Eph signals are particularly important in regulating cell adhesion and cell migration during development, axon guidance, homeostasis and disease. EphA receptors bind to GPI-anchored ephrin-A ligands, while EphB receptors bind to ephrin-B proteins that have a transmembrane and cytoplasmic domain. Interactions between EphB receptor kinases and ephrin-B proteins transduce signals bidirectionally, signaling to both interacting cell types. Eph receptors and ephrins also regulate the adhesion of endothelial cells and are required for the remodeling of blood vessels. Overexpressed in many cancers including aggressive ovarian, cervical and breast carcinomas, and lung cancer. Expression correlates with degree of angiogenesis, metastasis and xenograft tumor growth. Soluble receptor inhibits tumor growth and angiogenesis in mice.

Protein type: Kinase, protein; Membrane protein, integral; EC 2.7.10.1; Protein kinase, tyrosine (receptor); Protein kinase, TK; TK group; Eph family

Chromosomal Location of Human Ortholog: 1p36

Cellular Component: cell surface; focal adhesion; integral to plasma membrane; plasma membrane

Molecular Function: protein binding; ephrin receptor activity; transmembrane receptor protein tyrosine kinase activity; ATP binding

Biological Process: neural tube development; axon guidance; peptidyl-tyrosine phosphorylation; viral reproduction; multicellular organismal development; notochord formation; osteoclast differentiation; bone remodeling; regulation of blood vessel endothelial cell migration; regulation of cell adhesion mediated by integrin; protein kinase B signaling cascade; ephrin receptor signaling pathway; angiogenesis; cell adhesion; vasculogenesis; skeletal development; axial mesoderm formation; cell migration; negative regulation of protein kinase B signaling cascade; mammary gland epithelial cell proliferation; regulation of angiogenesis; keratinocyte differentiation; osteoblast differentiation; DNA damage response, signal transduction resulting in induction of apoptosis; notochord cell development

Disease: Cataract 6, Multiple Types