Rabbit GPCR RDC1/CXCR7 Monoclonal Antibody | anti-ACKR3 antibody

GPCR RDC1/CXCR7 Rabbit mAb

Cat. Num. AAA4752782

• Gene Names: CXCR7; RDC1; RDC-1; CMKOR1; CXC-R7; CXCR-7; GPR159
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Flow Cytometry, Functional Assay
• Purity: Affinity purified
• Synonyms: GPCR RDC1/CXCR7; Monoclonal Antibody; GPCR RDC1/CXCR7 Rabbit mAb; Atypical chemokine receptor 3; RDC1; CXCR7; RDC-1; CMKOR1; CXC-R7; CXCR-7; GPR159; anti-ACKR3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: Rabbit IgG
• Clone Number: R08-6H8
• Specificity: Expressed in monocytes, basophils, B-cells, umbilical vein endothelial cells (HUVEC) and B-lymphoblastoid cells. Lower expression detected in CD4+ T-lymphocytes and natural killer cells. In the brain, detected in endothelial cells and capillaries, and in mature neurons of the frontal cortex and hippocampus. Expressed in tubular formation in the kidney. Highly expressed in astroglial tumor endothelial, microglial and glioma cells. Expressed at low levels in normal CD34+ progenitor cells, but at very high levels in several myeloid malignant cell lines. Expressed in breast carcinomas but not in normal breast tissue (at protein level).
• Purity/Purification: Affinity purified
• Form/Format: Supplied in 50nM Tris-Glycine (pH7.4), 0.15M Nacl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

• Applicable Applications for anti-ACKR3 antibody: Western Blot (WB), Flow Cytometry (FC/FACS)
• Application Notes: WB: 1:1000-1:5000; FC/FACS: 1:20
• Immunogen: A synthetic peptide of human GPCR RDC1
• Conjugation: Unconjugated
• Modification: Unmodification
• Subcellular Location: Cell membrane, Cytoplasm, Endosome, Membrane
• Preparation and Storage: Store at 4 degree C short term. Aliquot and store at -20 degree C long term. Avoid freeze/thaw cycle.

Western Blot (WB)

(Western blot detection of GPCR RDC1/CXCR7 in K562,C6,3T3,Hela cell lysates using GPCR RDC1/CXCR7 Rabbit mAb(1:1000 diluted). Predicted band size:42kDa. Observed band size:42kDa.)

Related Product Information for anti-ACKR3 antibody

This gene encodes a member of the G-protein coupled receptor family. Although this protein was earlier thought to be a receptor for vasoactive intestinal peptide (VIP), it is now considered to be an orphan receptor, in that its endogenous ligand has not been identified. The protein is also a coreceptor for human immunodeficiency viruses (HIV). Translocations involving this gene and HMGA2 on chromosome 12 have been observed in lipomas. [provided by RefSeq, Jul 2008]
Atypical chemokine receptor that controls chemokine levels and localization via high-affinity chemokine binding that is uncoupled from classic ligand-driven signal transduction cascades, resulting instead in chemokine sequestration, degradation, or transcytosis. Also known as interceptor (internalizing receptor) or chemokine-scavenging receptor or chemokine decoy receptor. Acts as a receptor for chemokines CXCL11 and CXCL12/SDF1. Chemokine binding does not activate G-protein-mediated signal transduction but instead induces beta-arrestin recruitment, leading to ligand internalization and activation of MAPK signaling pathway. Required for regulation of CXCR4 protein levels in migrating interneurons, thereby adapting their chemokine responsiveness. In glioma cells, transduces signals via MEK/ERK pathway, mediating resistance to apoptosis. Promotes cell growth and survival. Not involved in cell migration, adhesion or proliferation of normal hematopoietic progenitors but activated by CXCL11 in malignant hemapoietic cells, leading to phosphorylation of ERK1/2 (MAPK3/MAPK1) and enhanced cell adhesion and migration. Plays a regulatory role in CXCR4-mediated activation of cell surface integrins by CXCL12. Required for heart valve development. Acts as coreceptor with CXCR4 for a restricted number of HIV isolates.

NCBI and Uniprot Product Information

• NCBI GI #: 31083344
• NCBI GeneID: 57007
• NCBI Accession #: NP_064707.1
• NCBI GenBank Nucleotide #: NM_020311.2
• UniProt Accession #: P25106; Q53RV4; Q8NE10; Q92938; Q92986; A8K6J4
• Molecular Weight: 41,493 Da
• NCBI Official Full Name: C-X-C chemokine receptor type 7
• NCBI Official Synonym Full Names: chemokine (C-X-C motif) receptor 7
• NCBI Official Symbol: CXCR7
• NCBI Official Synonym Symbols: RDC1; RDC-1; CMKOR1; CXC-R7; CXCR-7; GPR159
• NCBI Protein Information: C-X-C chemokine receptor type 7; G protein-coupled receptor; chemokine orphan receptor 1; G-protein coupled receptor 159; G-protein coupled receptor RDC1 homolog
• UniProt Protein Name: C-X-C chemokine receptor type 7
• Protein Family: Atypical chemokine receptor
• UniProt Gene Name: CXCR7
• UniProt Synonym Gene Names: CMKOR1; GPR159; RDC1; CXC-R7; CXCR-7; RDC-1
• UniProt Entry Name: CXCR7_HUMAN

NCBI Description

This gene encodes a member of the G-protein coupled receptor family. Although this protein was earlier thought to be a receptor for vasoactive intestinal peptide (VIP), it is now considered to be an orphan receptor, in that its endogenous ligand has not been identified. The protein is also a coreceptor for human immunodeficiency viruses (HIV). Translocations involving this gene and HMGA2 on chromosome 12 have been observed in lipomas. [provided by RefSeq, Jul 2008]

Uniprot Description

CMKOR1: Receptor for chemokines CXCL12/SDF1 and CXCL11. Does not elicit classical chemokine receptor signaling; chemokine binding does not activate G-protein-mediated signal transduction but instead induces beta-arrestin recruitment, leading to ligand internalization and activation of MAPK signaling pathway. Acts as a scavenger for CXCL12/SDF1 and, to a lesser extent, for CXCL11. Required for regulation of CXCR4 protein levels in migrating interneurons, thereby adapting their chemokine responsiveness. In glioma cells, transduces signals via MEK/ERK pathway, mediating resistance to apoptosis. Promotes cell growth and survival. Not involved in cell migration, adhesion or proliferation of normal hematopoietic progenitors but activated by CXCL11 in malignant hemapoietic cells, leading to phosphorylation of ERK1/2 (MAPK3/MAPK1) and enhanced cell adhesion and migration. Plays a regulatory role in CXCR4-mediated activation of cell surface integrins by CXCL12. Required for heart valve development. Acts as coreceptor with CXCR4 for a restricted number of HIV isolates. Belongs to the G-protein coupled receptor 1 family.

Protein type: GPCR, family 1; Receptor, GPCR; Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 2q37.3

Cellular Component: recycling endosome; cell surface; perinuclear region of cytoplasm; early endosome; plasma membrane; integral to membrane; coated pit; endosome

Molecular Function: C-X-C chemokine binding; protein binding; coreceptor activity; scavenger receptor activity; C-X-C chemokine receptor activity

Biological Process: G-protein coupled receptor protein signaling pathway; viral reproduction; receptor internalization; angiogenesis; cell adhesion; vasculogenesis; chemotaxis