Rabbit anti-Human EP / DBI Polyclonal Antibody | anti-EP antibody

Rabbit Polyclonal (IgG) to Human EP / DBI

Cat. Num. AAA244660

• Gene Names: DBI; EP; ACBP; ACBD1; CCK-RP
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, Immunoprecipitation
• Purity: Purified
• Synonyms: EP / DBI; Polyclonal Antibody; Rabbit Polyclonal (IgG) to Human EP / DBI; Anti-EP / DBI Antibody IHC-plus; DBI; ACBP; Acyl-CoA-binding protein; CCK-RP; Diazepam-binding inhibitor; ACBD1; GABA receptor modulator; EP; Endozepine; Human EP; anti-EP antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified
• Form/Format: HEPES with 0.15M NaCl, 0.01% BSA, 0.03% sodium azide, and 50% glycerol
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 104

• Applicable Applications for anti-EP antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: IHC-P (1:400), IP, WB (1:1000)
• Target Species: Human
• Immunogen Description: Recombinant human GST-fusion protein purified from E Coli.
• Immunogen Type: Recombinant protein
• Immunogen: EP / DBI antibody was raised against recombinant human GST-fusion protein purified from E Coli.
• Preparation and Storage: Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.

Immunohistochemistry (IHC)

(Anti-DBI antibody IHC of human spleen, lymphocytes. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:400.)

Western Blot (WB)

( )

NCBI and Uniprot Product Information

• NCBI GI #: 10140853
• NCBI GeneID: 1622
• NCBI Accession #: NP_065438.1
• NCBI GenBank Nucleotide #: NM_020548.7
• UniProt Accession #: P07108; P08869; Q4VWZ6; Q53SQ7; Q6IB48; Q9UCI8; B8ZWD2; B8ZWD6; B8ZWD7
• Molecular Weight: 13,911 Da
• NCBI Official Full Name: acyl-CoA-binding protein isoform 1
• NCBI Official Synonym Full Names: diazepam binding inhibitor (GABA receptor modulator, acyl-CoA binding protein)
• NCBI Official Symbol: DBI
• NCBI Official Synonym Symbols: EP; ACBP; ACBD1; CCK-RP
• NCBI Protein Information: acyl-CoA-binding protein; endozepine; GABA receptor modulator; diazepam-binding inhibitor; acyl coenzyme A binding protein; acyl-Coenzyme A binding domain containing 1; cholecystokinin-releasing peptide, trypsin-sensitive; diazepam binding inhibitor (GABA receptor modulator, acyl-Coenzyme A binding protein)
• UniProt Protein Name: Acyl-CoA-binding protein
• UniProt Gene Name: DBI
• UniProt Synonym Gene Names: ACBP; DBI; EP
• UniProt Entry Name: ACBP_HUMAN

NCBI Description

This gene encodes diazepam binding inhibitor, a protein that is regulated by hormones and is involved in lipid metabolism and the displacement of beta-carbolines and benzodiazepines, which modulate signal transduction at type A gamma-aminobutyric acid receptors located in brain synapses. The protein is conserved from yeast to mammals, with the most highly conserved domain consisting of seven contiguous residues that constitute the hydrophobic binding site for medium- and long-chain acyl-Coenzyme A esters. Diazepam binding inhibitor is also known to mediate the feedback regulation of pancreatic secretion and the postprandial release of cholecystokinin, in addition to its role as a mediator in corticotropin-dependent adrenal steroidogenesis. Three pseudogenes located on chromosomes 6, 8 and 16 have been identified. Multiple transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

DBI: Binds medium- and long-chain acyl-CoA esters with very high affinity and may function as an intracellular carrier of acyl-CoA esters. It is also able to displace diazepam from the benzodiazepine (BZD) recognition site located on the GABA type A receptor. It is therefore possible that this protein also acts as a neuropeptide to modulate the action of the GABA receptor. Belongs to the ACBP family. 6 isoforms of the human protein are produced by alternative promoter.

Protein type: Lipid-binding

Chromosomal Location of Human Ortholog: 2q12-q21

Cellular Component: Golgi apparatus; mitochondrion; endoplasmic reticulum

Molecular Function: protein dimerization activity; benzodiazepine receptor binding; lipid binding

Biological Process: triacylglycerol metabolic process; behavioral fear response; learning and/or memory; protein palmitoylation; hair follicle development; transport; lateral ventricle development