Western Blot (WB)
(Western blot analysis of ENPP7 Antibody (Center) in HL-60 cell line lysates (35ug/lane). ENPP7 (arrow) was detected using the purified Pab.)
Rabbit anti-Human ENPP7 Polyclonal Antibody | anti-ENPP7 antibody
ENPP7, ID (ENPP7, Ectonucleotide pyrophosphatase/phosphodiesterase family member 7, Alkaline sphingomyelin phosphodiesterase, Intestinal alkaline sphingomyelinase) (Biotin)
Gene Names
ENPP7; NPP7; NPP-7; E-NPP 7; ALK-SMase
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, ELISA, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
ENPP7; Polyclonal Antibody; ID (ENPP7; Ectonucleotide pyrophosphatase/phosphodiesterase family member 7; Alkaline sphingomyelin phosphodiesterase; Intestinal alkaline sphingomyelinase) (Biotin); Intestinal alkaline sphingomyelinase; anti-ENPP7 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Applicable Applications for anti-ENPP7 antibody
Flow Cytometry (FC/FACS), ELISA (EIA), Western Blot (WB)
Application Notes
FC/FACS: 1:10-50
ELISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
ELISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
Immunogen
ENPP7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between amino acid 290-320 from the Central region of human ENPP7.
Conjugate
Biotin
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western blot analysis of ENPP7 Antibody (Center) in HL-60 cell line lysates (35ug/lane). ENPP7 (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of ENPP7 Antibody (Center) in HL-60 cell line lysates (35ug/lane). ENPP7 (arrow) was detected using the purified Pab.)
Flow Cytometry (FC/FACS)
(ENPP7 Antibody (Center) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(ENPP7 Antibody (Center) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-ENPP7 antibody
ENPP7 converts sphingomyelin to ceramide. ENPP7 also has phospholipase C activity toward palmitoyl lyso-phosphocholine. ENPP7 does not appear to have nucleotide pyrophosphatase activity.
Product Categories/Family for anti-ENPP7 antibody
References
Wu, J., et al. Biochem. J. 386 (PT 1), 153-160 (2005). Zhang, Z., et al. Protein Sci. 13(10):2819-2824(2004). Wu, J., et al. Carcinogenesis 25(8):1327-1333(2004).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
458
NCBI Official Full Name
Ectonucleotide pyrophosphatase/phosphodiesterase family member 7
NCBI Official Synonym Full Names
ectonucleotide pyrophosphatase/phosphodiesterase 7
NCBI Official Symbol
ENPP7
NCBI Official Synonym Symbols
NPP7; NPP-7; E-NPP 7; ALK-SMase
NCBI Protein Information
ectonucleotide pyrophosphatase/phosphodiesterase family member 7; ENPP7; alkaline sphingomyelinase; intestinal alkaline sphingomyelinase; alkaline sphingomyelin phosphodiesterase
UniProt Protein Name
Ectonucleotide pyrophosphatase/phosphodiesterase family member 7
Protein Family
UniProt Gene Name
ENPP7
UniProt Synonym Gene Names
E-NPP 7; NPP-7; Alk-SMase
UniProt Entry Name
ENPP7_HUMAN
Uniprot Description
ENPP7: Converts sphingomyelin to ceramide. Also has phospholipase C activity toward palmitoyl lyso-phosphocholine. Does not appear to have nucleotide pyrophosphatase activity. Belongs to the nucleotide pyrophosphatase/phosphodiesterase family.
Protein type: Phosphodiesterase; Lipid Metabolism - sphingolipid; Cell cycle regulation; DNA replication; Motility/polarity/chemotaxis; Membrane protein, integral; EC 3.1.4.12
Chromosomal Location of Human Ortholog: 17q25.3
Cellular Component: Golgi apparatus; microvillus; membrane; integral to membrane; plasma membrane
Molecular Function: sphingomyelin phosphodiesterase activity
Biological Process: negative regulation of cell proliferation; sphingomyelin metabolic process; sphingolipid metabolic process; negative regulation of DNA replication; glycosphingolipid metabolic process; sphingomyelin catabolic process
Research Articles on ENPP7
Similar Products
Product Notes
The ENPP7 enpp7 (Catalog #AAA6295723) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ENPP7, ID (ENPP7, Ectonucleotide pyrophosphatase/phosphodiesterase family member 7, Alkaline sphingomyelin phosphodiesterase, Intestinal alkaline sphingomyelinase) (Biotin) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ENPP7 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), ELISA (EIA), Western Blot (WB). FC/FACS: 1:10-50 ELISA: 1:1,000 WB: 1:100-500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the ENPP7 enpp7 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ENPP7, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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