Rabbit anti-Human Enolase 3 Polyclonal Antibody | anti-ENO3 antibody

Enolase 3 antibody

Cat. Num. AAA9410072

• Gene Names: ENO3; MSE; GSD13
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry
• Purity: Purified by antigen-affinity chromatography.
• Synonyms: Enolase 3; Polyclonal Antibody; Enolase 3 antibody; anti-ENO3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Purity/Purification: Purified by antigen-affinity chromatography.
• Form/Format: Supplied in 0.1M Tris-buffered saline with 10% Glycerol (pH7.0). 0.01% Thimerosal was added as a preservative
• Concentration: 0.46mg/ml (varies by lot)
• Sequence Length: 434

• Applicable Applications for anti-ENO3 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Western blotting: 1:500-1:3000; Immunohistochemistry: 1:100-1:500
• Immunogen Type: Recombinant protein
• Immunogen Description: Recombinant protein fragment contain a sequence corresponding to a region within amino acids 196 and 413 of human ENO3
• Target Name: Enolase 3
• Preparation and Storage: Store at -20 degree C for long term preservation (recommended). Store at 4 degree C for short term use.

Testing Data

(Sample (30 ug of whole cell lysate) A: Hep G2 B: Molt-4 C: Raji 10% SDS PAGE Primary antibody diluted at 1: 1000)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using ENO3 antibody at 1: 500 dilution.)

Related Product Information for anti-ENO3 antibody

This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in skeletal muscle cells in the adult. A switch from alpha enolase to beta enolase occurs in muscle tissue during development in rodents. Mutations in this gene can be associated with metabolic myopathies that may result from decreased stability of the enzyme. Two transcripts have been identified for this gene that differ only in their 5' UTR. [provided by RefSeq]

Product Categories/Family for anti-ENO3 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 16878083
• NCBI GeneID: 2027
• Molecular Weight: 47kd
• NCBI Official Full Name: Enolase 3 (beta, muscle)
• NCBI Official Synonym Full Names: enolase 3 (beta, muscle)
• NCBI Official Symbol: ENO3
• NCBI Official Synonym Symbols: MSE; GSD13
• NCBI Protein Information: beta-enolase
• UniProt Protein Name: Beta-enolase
• Protein Family: Enolase
• UniProt Gene Name: ENO3
• UniProt Synonym Gene Names: MSE
• UniProt Entry Name: ENOB_HUMAN

NCBI Description

This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme is found in skeletal muscle cells in the adult where it may play a role in muscle development and regeneration. A switch from alpha enolase to beta enolase occurs in muscle tissue during development in rodents. Mutations in this gene have be associated glycogen storage disease. Alternatively spliced transcript variants encoding different isoforms have been described.[provided by RefSeq, Jul 2010]

Uniprot Description

ENO3: Appears to have a function in striated muscle development and regeneration. Defects in ENO3 are the cause of glycogen storage disease type 13 (GSD13). A metabolic disorder that results in exercise-induced myalgias, generalized muscle weakness and fatigability. It is characterized by increased serum creatine kinase and decreased enolase 3 activity. Dramatically reduced protein levels with focal sarcoplasmic accumulation of glycogen- beta particles are detected on ultrastructural analysis. Belongs to the enolase family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Carbohydrate Metabolism - glycolysis and gluconeogenesis; EC 4.2.1.11; Lyase

Chromosomal Location of Human Ortholog: 17p13.2

Cellular Component: extracellular space; phosphopyruvate hydratase complex; cytoplasm; plasma membrane; cytosol

Molecular Function: protein homodimerization activity; protein heterodimerization activity; magnesium ion binding; phosphopyruvate hydratase activity

Biological Process: response to drug; glycolysis; carbohydrate metabolic process; glucose metabolic process; skeletal muscle regeneration; pathogenesis; aging; gluconeogenesis

Disease: Glycogen Storage Disease Xiii