Testing Data
(Sample (30 ug of whole cell lysate) A: A431 B: H1299 10% SDS PAGE Primary antibody diluted at 1: 3000)
Rabbit anti-Human enolase 1 Polyclonal Antibody | anti-ENO1 antibody
enolase 1 antibody
Immunohistochemistry: 1:100-1:250
Immunofluorescence: 1:100-1:200
Testing Data
(Sample (30 ug of whole cell lysate) A: A431 B: H1299 10% SDS PAGE Primary antibody diluted at 1: 3000)
Testing Data
(Sample (30 ug of whole cell lysate) A: A431 B: H1299 10% SDS PAGE Primary antibody diluted at 1: 3000)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded gastric tissue, using ENO1 antibody at 1: 100 dilution.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded gastric tissue, using ENO1 antibody at 1: 100 dilution.)
Immunofluorescence (IF)
(Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using enolase 1 antibody at 1: 200 dilution.)
Immunofluorescence (IF)
(Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using enolase 1 antibody at 1: 200 dilution.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes alpha-enolase, one of three enolase isoenzymes found in mammals. Each isoenzyme is a homodimer composed of 2 alpha, 2 gamma, or 2 beta subunits, and functions as a glycolytic enzyme. Alpha-enolase in addition, functions as a structural lens protein (tau-crystallin) in the monomeric form. Alternative splicing of this gene results in a shorter isoform that has been shown to bind to the c-myc promoter and function as a tumor suppressor. Several pseudogenes have been identified, including one on the long arm of chromosome 1. Alpha-enolase has also been identified as an autoantigen in Hashimoto encephalopathy. [provided by RefSeq, Jan 2011]
Uniprot Description
ENO1: an abundant cytoplasmic enzyme with 2-phospho-D-glycerate hydro-lyase activity. A target of excess protein nitration in Alzheimer disease (AD) brain. Tau-crystallin, a structural lens protein, is produced from an alternative translation start in the enolase gene.
Protein type: Carbohydrate Metabolism - glycolysis and gluconeogenesis; Lyase; EC 4.2.1.11; Transcription, coactivator/corepressor
Chromosomal Location of Human Ortholog: 1p36.2
Cellular Component: extracellular space; phosphopyruvate hydratase complex; membrane; cytoplasm; plasma membrane; M band; cytosol; nucleus
Molecular Function: protein binding; DNA binding; magnesium ion binding; phosphopyruvate hydratase activity; transcription corepressor activity; transcription factor activity
Biological Process: glycolysis; transcription, DNA-dependent; response to virus; carbohydrate metabolic process; glucose metabolic process; pathogenesis; negative regulation of cell growth; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; gluconeogenesis
Research Articles on ENO1
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Product Notes
The ENO1 eno1 (Catalog #AAA9410123) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The enolase 1 antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's enolase 1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). Western blotting: 1:500-1:3000 Immunohistochemistry: 1:100-1:250 Immunofluorescence: 1:100-1:200. Researchers should empirically determine the suitability of the ENO1 eno1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "enolase 1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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