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Immunohistochemistry (IHC) (Anti-ENO2 / NSE antibody IHC staining of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.)

Chicken anti-Rat, Human ENO2 / NSE Polyclonal Antibody | anti-ENO2 / NSE antibody

Anti-ENO2 / NSE Antibody IHC-plus

Gene Names
ENO2; NSE; HEL-S-279
Reactivity
Rat, Human
Applications
Immunohistochemistry, Immunocytochemistry, Western Blot
Purity
Affinity purified
Synonyms
ENO2 / NSE; Polyclonal Antibody; Anti-ENO2 / NSE Antibody IHC-plus; Chicken Polyclonal (IgY); ENO2; Enolase 2; Enolase 2 (gamma; neuronal); Neurone-specific enolase; Gamma-enolase; Neural enolase; Neuron specific gamma enolase; Neuron-specific enolase; NSE; anti-ENO2 / NSE antibody
Ordering
For Research Use Only!
Host
Chicken
Reactivity
Rat, Human
Clonality
Polyclonal
Isotype
IgY
Specificity
These synthetic peptides corresponded to different regions of the NSE-2 gene product, but are shared between the human (NP_001966, NCBI) and rat (AAA41119, NCBI) sequences.
Purity/Purification
Affinity purified
Form/Format
Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline ("PBS," pH 7.2) with sodium azide (0.02%, w/v) added as a preservative.
Sequence Length
434
Applicable Applications for anti-ENO2 / NSE antibody
Immunohistochemistry (IHC) Paraffin, Immunocytochemistry (ICC), Western Blot (WB)
Application Notes
ICC (1:2000 - 1:5000)
IHC-P (1:500)
WB (1:5000 - 1:10000)
Immunogen
ENO2 / NSE antibody was raised against two synthetic peptide / keyhole limpet hemocyanin
Immunogen Description
Two synthetic peptide / keyhole limpet hemocyanin
Immunogen Type
Synthetic peptide
Preparation and Storage
May be stored at 4 degree C. For long-term storage, aliquot and store at 4 degree C. Do not freeze. Aliquots are stable for at least 12 months. Sensitive to light.

Immunohistochemistry (IHC)

(Anti-ENO2 / NSE antibody IHC staining of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.)

Immunohistochemistry (IHC) (Anti-ENO2 / NSE antibody IHC staining of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.)
Related Product Information for anti-ENO2 / NSE antibody
ENO2 Antibody, Enolase 2 Antibody, Enolase 2 (gamma, neuronal) Antibody, Neurone-specific enolase Antibody, Gamma-enolase Antibody, Neural enolase Antibody, Neuron specific gamma enolase Antibody, Neuron-specific enolase Antibody, NSE Antibody Description: This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
42,707 Da
NCBI Official Full Name
gamma-enolase
NCBI Official Synonym Full Names
enolase 2 (gamma, neuronal)
NCBI Official Symbol
ENO2
NCBI Official Synonym Symbols
NSE; HEL-S-279
NCBI Protein Information
gamma-enolase
UniProt Protein Name
Gamma-enolase
UniProt Gene Name
ENO2
UniProt Synonym Gene Names
NSE
UniProt Entry Name
ENOG_HUMAN

NCBI Description

This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates. [provided by RefSeq, Jul 2008]

Uniprot Description

ENO2: an enzyme with 2-phospho-D-glycerate hydro-lyase activity. One of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates.

Protein type: EC 4.2.1.11; Lyase; Carbohydrate Metabolism - glycolysis and gluconeogenesis

Chromosomal Location of Human Ortholog: 12p13

Cellular Component: cytosol; extracellular space; myelin sheath; perikaryon; phosphopyruvate hydratase complex; photoreceptor inner segment; plasma membrane

Molecular Function: magnesium ion binding; phosphopyruvate hydratase activity; protein binding

Biological Process: carbohydrate metabolic process; gluconeogenesis; glucose metabolic process

Research Articles on ENO2 / NSE

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Product Notes

The ENO2 / NSE eno2 (Catalog #AAA249623) is an Antibody produced from Chicken and is intended for research purposes only. The product is available for immediate purchase. The Anti-ENO2 / NSE Antibody IHC-plus reacts with Rat, Human and may cross-react with other species as described in the data sheet. AAA Biotech's ENO2 / NSE can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC) Paraffin, Immunocytochemistry (ICC), Western Blot (WB). ICC (1:2000 - 1:5000) IHC-P (1:500) WB (1:5000 - 1:10000). Researchers should empirically determine the suitability of the ENO2 / NSE eno2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ENO2 / NSE, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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