Western Blot (WB)
(ENO1 Antibody (Center) western blot analysis in Jurkat,MCF-7,U251,mouse NIH/3T3 cell line and mouse brain tissue lysates (35ug/lane).This demonstrates the ENO1 antibody detected the ENO1 protein (arrow).)
Rabbit anti-Human ENO1 Polyclonal Antibody | anti-ENO1 antibody
ENO1, ID (Alpha-enolase, 2-phospho-D-glycerate Hydro-lyase, Non-neural Enolase, NNE, Enolase 1, Phosphopyruvate Hydratase, C-myc Promoter-binding Protein, MBP-1, MPB-1, Plasminogen-binding Protein, MPB1, MBPB1, ENO1L1, ENO1) (MaxLight 405)
FLISA: 1:1,000
Applications are based on unconjugated antibody.
Western Blot (WB)
(ENO1 Antibody (Center) western blot analysis in Jurkat,MCF-7,U251,mouse NIH/3T3 cell line and mouse brain tissue lysates (35ug/lane).This demonstrates the ENO1 antibody detected the ENO1 protein (arrow).)
Western Blot (WB)
(ENO1 Antibody (Center) western blot analysis in Jurkat,MCF-7,U251,mouse NIH/3T3 cell line and mouse brain tissue lysates (35ug/lane).This demonstrates the ENO1 antibody detected the ENO1 protein (arrow).)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human lymph reacted with ENO1 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human lymph reacted with ENO1 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS)
(ENO1 Antibody (Center) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(ENO1 Antibody (Center) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)
Immunofluorescence (IF)
(Fluorescent confocal image of C2C12 cell stained with ENO1 Antibody (Center).C2C12 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ENO1 primary antibody (1:25, 1 h at 37 degree C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). ENO1 immunoreactivity is localized to Cytoplasm significantly.)
Immunofluorescence (IF)
(Fluorescent confocal image of C2C12 cell stained with ENO1 Antibody (Center).C2C12 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ENO1 primary antibody (1:25, 1 h at 37 degree C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). ENO1 immunoreactivity is localized to Cytoplasm significantly.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes alpha-enolase, one of three enolase isoenzymes found in mammals. Each isoenzyme is a homodimer composed of 2 alpha, 2 gamma, or 2 beta subunits, and functions as a glycolytic enzyme. Alpha-enolase in addition, functions as a structural lens protein (tau-crystallin) in the monomeric form. Alternative splicing of this gene results in a shorter isoform that has been shown to bind to the c-myc promoter and function as a tumor suppressor. Several pseudogenes have been identified, including one on the long arm of chromosome 1. Alpha-enolase has also been identified as an autoantigen in Hashimoto encephalopathy. [provided by RefSeq, Jan 2011]
Research Articles on ENO1
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Product Notes
The ENO1 eno1 (Catalog #AAA6474041) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ENO1, ID (Alpha-enolase, 2-phospho-D-glycerate Hydro-lyase, Non-neural Enolase, NNE, Enolase 1, Phosphopyruvate Hydratase, C-myc Promoter-binding Protein, MBP-1, MPB-1, Plasminogen-binding Protein, MPB1, MBPB1, ENO1L1, ENO1) (MaxLight 405) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ENO1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), FLISA. WB: 1:50-1:100 FLISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the ENO1 eno1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ENO1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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