Rabbit anti-Human Endothelial Monocyte Activating Polypeptide II Polyclonal Antibody | anti-EMAP2 antibody

Endothelial Monocyte Activating Polypeptide II, CT (EMAP II, EMAPII, Endothelial Monocyte Activating Polypeptide 2, EMAP 2, EMAP2, Aminoacyl tRNA Synthase Complex-interacting Multifunctional Protein 1, AIMP1, Multisynthetase Complex Auxiliary Component p4

Cat. Num. AAA6473980

• Gene Names: AIMP1; p43; HLD3; EMAP2; SCYE1; EMAPII
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: Endothelial Monocyte Activating Polypeptide II; Polyclonal Antibody; CT (EMAP II; EMAPII; Endothelial Monocyte Activating Polypeptide 2; EMAP 2; EMAP2; Aminoacyl tRNA Synthase Complex-interacting Multifunctional Protein 1; AIMP1; Multisynthetase Complex Auxiliary Component p4; MCA1; anti-EMAP2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human MCA1.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-EMAP2 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:50-1:100; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human MCA1 (KLH).
• Conjugate: PE
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of MCA1 antibody (C-term) in 293 cell line lysates (35ug/lane). MCA1 (arrow) was detected using the purified Pab.)

Western Blot (WB)

(Western blot analysis of MCA1(arrow) using rabbit polyclonal MCA1 Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MCA1 gene (Lane 2) (Origene Technologies).)

Immunohistochemistry (IHC)

(MCA1 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human lymphnode followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the MCA1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Product Categories/Family for anti-EMAP2 antibody

Antibodies; Growth Factors; Cytokines

References

1. Awasthi, N., Lab. Invest. 89(1), 38-46 (2009). 2. Sen, E., Clin Lung Cancer 9(3), 166-170 (2008).

NCBI and Uniprot Product Information

• NCBI GI #: 45006986
• NCBI GeneID: 9255
• NCBI Accession #: NP_004748
• NCBI GenBank Nucleotide #: NM_004757
• UniProt Accession #: Q12904
• Molecular Weight: 34kDa
• NCBI Official Full Name: aminoacyl tRNA synthase complex-interacting multifunctional protein 1 isoform a
• NCBI Official Synonym Full Names: aminoacyl tRNA synthetase complex interacting multifunctional protein 1
• NCBI Official Symbol: AIMP1
• NCBI Official Synonym Symbols: p43; HLD3; EMAP2; SCYE1; EMAPII
• NCBI Protein Information: aminoacyl tRNA synthase complex-interacting multifunctional protein 1
• UniProt Protein Name: Aminoacyl tRNA synthase complex-interacting multifunctional protein 1
• UniProt Gene Name: AIMP1
• UniProt Synonym Gene Names: EMAP2; SCYE1; EMAP-2; EMAP-II
• UniProt Entry Name: AIMP1_HUMAN

NCBI Description

The protein encoded by this gene is a cytokine that is specifically induced by apoptosis, and it is involved in the control of angiogenesis, inflammation, and wound healing. The release of this cytokine renders the tumor-associated vasculature sensitive to tumor necrosis factor. The precursor protein is identical to the p43 subunit, which is associated with the multi-tRNA synthetase complex, and it modulates aminoacylation activity of tRNA synthetase in normal cells. This protein is also involved in the stimulation of inflammatory responses after proteolytic cleavage in tumor cells. Multiple transcript variants encoding different isoforms have been found for this gene. A pseudogene has been identified on chromosome 20. [provided by RefSeq, Dec 2008]

Uniprot Description

SCYE1 iso2: Non-catalytic component of the multisynthase complex. Stimulates the catalytic activity of cytoplasmic arginyl-tRNA synthase. Binds tRNA. Possesses inflammatory cytokine activity. Negatively regulates TGF-beta signaling through stabilization of SMURF2 by binding to SMURF2 and inhibiting its SMAD7-mediated degradation. Involved in glucose homeostasis through induction of glucagon secretion at low glucose levels. Promotes dermal fibroblast proliferation and wound repair. Regulates KDELR1- mediated retention of HSP90B1/gp96 in the endoplasmic reticulum. Plays a role in angiogenesis by inducing endothelial cell migration at low concentrations and endothelian cell apoptosis at high concentrations. Induces maturation of dendritic cells and monocyte cell adhesion. Modulates endothelial cell responses by degrading HIF-1A through interaction with PSMA7. Defects in AIMP1 are the cause of leukodystrophy hypomyelinating type 3 (HLD3). A severe autosomal recessive hypomyelinating leukodystrophy characterized by early infantile onset of global developmental delay, lack of development, lack of speech acquisition, and peripheral spasticity associated with decreased myelination in the central nervous system. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 4q24

Cellular Component: Golgi apparatus; extracellular space; aminoacyl-tRNA synthetase multienzyme complex; transport vesicle; cell surface; membrane; endoplasmic reticulum; cytoplasm; nucleus; cytosol

Molecular Function: methionine-tRNA ligase activity; protein binding; protein homodimerization activity; cytokine activity; tRNA binding

Biological Process: tRNA aminoacylation for protein translation; cell-cell signaling; apoptosis; negative regulation of endothelial cell proliferation; response to wounding; methionyl-tRNA aminoacylation; glucose metabolic process; gene expression; angiogenesis; signal transduction; inflammatory response; chemotaxis; cell adhesion; leukocyte migration

Disease: Leukodystrophy, Hypomyelinating, 3