Endoplasmic Reticulum Associated Amyloid Beta Binding Protein Polyclonal Antibody

Anti - Endoplasmic Reticulum Associated Amyloid Beta Binding Protein (ERAB)

Cat. Num. AAA618377

• Reactivity: Bovine, Human, Mouse
• Applications: ELISA, Western Blot
• Purity: Purified by immunoaffinity chromatography.
• Synonyms: Endoplasmic Reticulum Associated Amyloid Beta Binding Protein; Polyclonal Antibody; Anti - Endoplasmic Reticulum Associated Amyloid Beta Binding Protein (ERAB); Endoplasmic Reticulum Associated Amyloid Beta Binding Protein (ERAB); anti-Endoplasmic Reticulum Associated Amyloid Beta Binding Protein antibody

• Host: Host: Rabbit; Source: Human
• Reactivity: Bovine, Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: The 17 AA human sequences is 70% (12/17 AA) homologous with mouse ERAB (1). It also has 100% homology with the human probable short chain type alcohol-dehydrogenase/reductase and bovine 3-hydroxyacyl-CoA dehydrogenase (2
• Purity/Purification: Purified by immunoaffinity chromatography.
• Form/Format: Affinity Purified; Supplied as a liquid in PBS, pH 7.4, 0.1% BSA, 0.02% sodium merthiolate, 40% glycerol.
• Concentration: 1 mg/ml (varies by lot)

• Applicable Applications for anti-Endoplasmic Reticulum Associated Amyloid Beta Binding Protein antibody: ELISA, Western Blot
• Application Notes: Suitable for use in ELISA and Western Blot. Other applications not tested.; Dilution: Western Blot: 1:1000-1:5000 for neat serum and 1-10ug/ml for affinity pure using ECL. An antibody made to the ERAB11 epitope has detected ~ 27kD protein in the brain.; ELISA: 1:10,000-1:100,000 using 50-100ng of control peptide/well.; Optimal dilutions to be determined by the researcher.
• Immunogen: A 17 amino acid peptide sequence near the amino terminus of human ERAB was used to generate anti-ERAB. The peptide was coupled with KLH (carrier protein)
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-Endoplasmic Reticulum Associated Amyloid Beta Binding Protein antibody

An intracellular protein termed ERAB (Endoplasmic reticulum associated Ab binding protein on chromosome X) has been cloned and linked with Ab neurotoxicity (1). ERAB has structural homology with short-chain alcohol dehydrogenases (hydroxysteroid dehydrogenases and acetyl-CoA reductases). Interestingly, ERAB localizes to endoplasmic reticulum despite the absence of a classical transmembrane sequence. However, in the presence of Ab, ERAB- Ab complex translates to the inner plasma membrane. Alzheimer patients have strong ERAB activity. Normal brains have virtually no ERAB. In addition, Ab neurotoxicity can be reduced by blocking ERAB and increased by its overexpression.

Product Categories/Family for anti-Endoplasmic Reticulum Associated Amyloid Beta Binding Protein antibody

Abs to Amyloid

References

• 1. Yan SD et al (1997) Nature 389:689.
• 2. Zuchenko OP et al (1997) Accession # Q99717; Furuta S et al (1997) BBA 1350:317-324.