Rabbit anti-Human ENAH Polyclonal Antibody | anti-ENAH antibody

ENAH, CT (ENAH, MENA, Protein enabled homolog) (AP)

Cat. Num. AAA6295523

• Gene Names: ENAH; ENA; MENA; NDPP1
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: ENAH; Polyclonal Antibody; CT (ENAH; MENA; Protein enabled homolog) (AP); Protein enabled homolog; anti-ENAH antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
• Sequence Length: 570

• Applicable Applications for anti-ENAH antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: ENAH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 446-475 amino acids from the C-terminal region of human ENAH.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(ENAH Antibody (C-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the ENAH antibody detected the ENAH protein (arrow).)

Related Product Information for anti-ENAH antibody

Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance and lamellipodial and filopodial dynamics in migrating cells. ENAH induces the formation of F-actin rich outgrowths in fibroblasts. Acts synergistically with BAIAP2-alpha and downstream of NTN1 to promote filipodia formation. Required for the actin-based mobility of Listeria monocytogenes (By similarity).

Product Categories/Family for anti-ENAH antibody

Antibodies; Proteins

References

Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010): Chattopadhyay, I., et al. Mutat. Res. 696(2):130-138(2010). Hahn, W.H., et al. Exp. Mol. Med. 41(11):793-801(2009). Gurzu, S., et al. Rom J Morphol Embryol 50(2):213-216(2009). Higashi, M., et al. PLoS ONE 4 (3), E4765 (2009).

NCBI and Uniprot Product Information

• NCBI GI #: 39930375
• NCBI GeneID: 55740
• NCBI Accession #: NP_060682.2
• NCBI GenBank Nucleotide #: NP_060682.2
• UniProt Accession #: Q8N8S7
• NCBI Official Full Name: protein enabled homolog isoform b
• NCBI Official Synonym Full Names: ENAH actin regulator
• NCBI Official Symbol: ENAH
• NCBI Official Synonym Symbols: ENA; MENA; NDPP1
• NCBI Protein Information: protein enabled homolog
• UniProt Protein Name: Protein enabled homolog
• Protein Family: Protein enabled
• UniProt Gene Name: ENAH
• UniProt Synonym Gene Names: MENA
• UniProt Entry Name: ENAH_HUMAN

NCBI Description

This gene encodes a member of the enabled/ vasodilator-stimulated phosphoprotein. Members of this gene family are involved in actin-based motility. This protein is involved in regulating the assembly of actin filaments and modulates cell adhesion and motility. Alternate splice variants of this gene have been correlated with tumor invasiveness in certain tissues and these variants may serve as prognostic markers. A pseudogene of this gene is found on chromosome 3. [provided by RefSeq, Sep 2016]

Uniprot Description

Mena: Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance and lamellipodial and filopodial dynamics in migrating cells. ENAH induces the formation of F-actin rich outgrowths in fibroblasts. Acts synergistically with BAIAP2-alpha and downstream of NTN1 to promote filipodia formation. Required for the actin-based mobility of Listeria monocytogenes. Homotetramer. Interacts with APBB1IP, APBB1, PFN1 and ROBO4. Isoforms, containing the polyproline-rich regions with PPLP motifs, bind the WW domain of APBB1IP. Isoforms, containing the PPSY motif, bind, in vitro, to the WW2 and WW3 domains of NEDD4 and to the WW1 domain of YAP1. Binds the SH3 domain of BAIAP2-alpha but only after the autoinhibitory region of BAIAP2-alpha has been blocked by interaction with CDC42. Interacts, via the EVH1/WH1 domain, with the Pro-rich domains from VCL, ZYX and Listeria monocytogenes actA and with TES (via LIM domains). The TES LIM domain and the Pro-rich domains from VCL or ZYX compete for the same binding site. Interaction with ZYX is important for targeting ENAH to focal adhesions and enhances production of actin-rich structures at the apical surface of cells. Interacts, through the Pro-rich region, with the C-terminal SH3 domain of DNMPB. Binds GPHN. Interacts with FAT1 (via EVH1 domains). Heterotrimer with TES and ACTL7A. Expressed in myoepithelia of parotid, breast, bronchial glands and sweat glands. Expressed in colon-rectum muscolaris mucosae epithelium, pancreas acinar ductal epithelium, endometrium epithelium, prostate fibromuscolar stroma and placenta vascular media. Overexpressed in a majority of breast cancer cell lines and primary breast tumor lesions. Belongs to the Ena/VASP family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 1q42.12

Cellular Component: focal adhesion; lamellipodium; cytoplasm; stress fiber; plasma membrane; synapse; cytosol; cell junction; filopodium

Molecular Function: protein binding; WW domain binding; actin binding; SH3 domain binding

Biological Process: axon guidance; intracellular transport; actin polymerization and/or depolymerization; neural tube closure; T cell receptor signaling pathway