Lysyl Oxidase (LOX) Protein | LOX protein

Eukaryotic Lysyl Oxidase (LOX)

Cat. Num. AAA2124332

• Applications: SDS-Page, Western Blot
• Purity: >90%
• Synonyms: Lysyl Oxidase (LOX); Eukaryotic Lysyl Oxidase (LOX); Lysyl Oxidase; Protein-Lysine 6-Oxidase; LOX protein

• Host: 293F cells
• Purity/Purification: >90%
• Form/Format: Freeze-dried powder; 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Sequence: Asp169~Tyr417

• Applicable Applications for LOX protein: Positive Control, Immunogen, SDS-PAGE, Western Blot (WB)
• Source: Eukaryotic expression
• Species: Human
• Tag: N-terminal His Tag
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Isoelectric Point: 6.3
• Subcellular Location: Secreted
• Usage: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
• Preparation and Storage: Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months. Avoid repeated freeze/thaw cycles.; The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Sequence Information

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SDS-Page

(Figure. SDS-PAGE)

Product Categories/Family for LOX protein

Enzyme & Kinase; Eukaryotic proteins/EP

NCBI and Uniprot Product Information

• NCBI GI #: 257065
• NCBI GeneID: 4015
• UniProt Accession #: P28300; Q5FWF0; B2R5Q3
• Molecular Weight: 46,944 Da
• NCBI Official Full Name: lysyl oxidase
• NCBI Official Synonym Full Names: lysyl oxidase
• NCBI Official Symbol: LOX
• NCBI Protein Information: protein-lysine 6-oxidase
• UniProt Protein Name: Protein-lysine 6-oxidase
• Protein Family: Linoleate 9/13-lipoxygenase
• UniProt Gene Name: LOX
• UniProt Entry Name: LYOX_HUMAN

NCBI Description

The protein encoded by this gene is an extracellular copper enzyme that initiates the crosslinking of collagens and elastin. The enzyme catalyzes oxidative deamination of the epsilon-amino group in certain lysine and hydroxylysine residues of collagens and lysine residues of elastin. In addition to crosslinking extracellular matrix proteins, the encoded protein may have a role in tumor suppression. Defects in this gene are a cause of autosomal recessive cutis laxa type I (CL type I). Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2010]

Uniprot Description

LOX: Responsible for the post-translational oxidative deamination of peptidyl lysine residues in precursors to fibrous collagen and elastin. In addition to cross-linking of extracellular matrix proteins, may have a direct role in tumor suppression. Belongs to the lysyl oxidase family.

Protein type: Secreted, signal peptide; EC 1.4.3.13; Secreted; Oxidoreductase

Chromosomal Location of Human Ortholog: 5q23.2

Cellular Component: proteinaceous extracellular matrix; extracellular space; collagen; extracellular region; nucleus

Molecular Function: protein binding; copper ion binding; protein-lysine 6-oxidase activity

Biological Process: response to drug; elastic fiber assembly; blood vessel development; extracellular matrix organization and biogenesis; collagen fibril organization; wound healing; response to steroid hormone stimulus; protein modification process; lung development

Disease: Cutis Laxa, Autosomal Recessive, Type Ia