Immunohistochemistry (IHC)
(Anti-EN1 / Engrailed antibody IHC staining of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)
Rabbit anti-Human EN1 / Engrailed Polyclonal Antibody | anti-EN1 antibody
Rabbit Polyclonal to Human EN1 / Engrailed
Immunohistochemistry (IHC)
(Anti-EN1 / Engrailed antibody IHC staining of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)
Immunohistochemistry (IHC)
(Anti-EN1 / Engrailed antibody IHC staining of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with EN1 antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with EN1 antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Western Blot (WB)
(Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with EN1 antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.)
Western Blot (WB)
(Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with EN1 antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.)
Western Blot (WB)
(Western blot of anti-EN1 Antibody (N-term) in NCI-H460 cell line lysates (35 ug/lane). EN1(arrow) was detected using the purified Pab (1:60 dilution).)
Western Blot (WB)
(Western blot of anti-EN1 Antibody (N-term) in NCI-H460 cell line lysates (35 ug/lane). EN1(arrow) was detected using the purified Pab (1:60 dilution).)
NCBI and Uniprot Product Information
NCBI Description
Homeobox-containing genes are thought to have a role in controlling development. In Drosophila, the 'engrailed' (en) gene plays an important role during development in segmentation, where it is required for the formation of posterior compartments. Different mutations in the mouse homologs, En1 and En2, produced different developmental defects that frequently are lethal. The human engrailed homologs 1 and 2 encode homeodomain-containing proteins and have been implicated in the control of pattern formation during development of the central nervous system. [provided by RefSeq, Jul 2008]
Uniprot Description
EN1: Homeobox-containing genes are thought to have a role in controlling development. In Drosophila, the 'engrailed' (en) gene plays an important role during development in segmentation, where it is required for the formation of posterior compartments. Different mutations in the mouse homologs, En1 and En2, produced different developmental defects that frequently are lethal. The human engrailed homologs 1 and 2 encode homeodomain-containing proteins and have been implicated in the control of pattern formation during development of the central nervous system. [provided by RefSeq, Jul 2008]
Protein type: DNA-binding; Transcription factor; Cell development/differentiation
Chromosomal Location of Human Ortholog: 2q14.2
Cellular Component: membrane; nucleus
Biological Process: embryonic forelimb morphogenesis; anatomical structure morphogenesis; multicellular organism growth; negative regulation of transcription from RNA polymerase II promoter; response to cocaine; pigmentation; dorsal/ventral pattern formation; midbrain-hindbrain boundary development; midbrain development; neuron development; positive regulation of transcription from RNA polymerase II promoter; skeletal development; proximal/distal pattern formation; hindbrain development
Research Articles on EN1
Similar Products
Product Notes
The EN1 en1 (Catalog #AAA248325) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit Polyclonal to Human EN1 / Engrailed reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's EN1 / Engrailed can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB). IF (1:10 - 1:50), IHC-P (10 ug/ml), WB (1:1000). Researchers should empirically determine the suitability of the EN1 en1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EN1 / Engrailed, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
