Mouse anti-Human Progesterone Receptor Monoclonal Antibody | anti-PGR antibody

Progesterone Receptor (NR3C3, PGR) (HRP)

Cat. Num. AAA6122421

• Gene Names: PGR; PR; NR3C3
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein G Affinity Chromatography
• Synonyms: Progesterone Receptor; Monoclonal Antibody; Progesterone Receptor (NR3C3; PGR) (HRP); anti-PGR antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: PR484
• Specificity: Recognizes human progesterone receptor.
• Purity/Purification: Purified by Protein G Affinity Chromatography
• Form/Format: Supplied as a liquid in 10mM PBS. No preservative added. BSA-Free. Labeled with horseradish peroxidase (HRP).

• Applicable Applications for anti-PGR antibody: Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: WB: 0.5-1ug/ml; Applications are based on unconjugated antibody.
• Immunogen: Recombinant protein corresponding to human progesterone receptor.
• Conjugate: HRP
• Positive Control: T47-D Cells or Breast Cancers.
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded normal human breast (left) and endometrial carcinoma (right) stained with Progesterone Receptor antibody (1:500), peroxidase-conjugate and DAB chromogen.)

Product Categories/Family for anti-PGR antibody

Antibodies; Receptors

References

1. Press M, et al. Steroids. 2002 Aug; 67(9): 799-813. 2. Mote P, et al. J Clin Pathol., 2001; 54: 624-630.

NCBI and Uniprot Product Information

• NCBI GI #: 110611914
• NCBI GeneID: 5241
• NCBI Accession #: NP_000917
• NCBI GenBank Nucleotide #: NM_000926
• UniProt Accession #: P06401; Q8TDS3; Q9UPF7; A7LQ08; A7X8B0; B4E3T0
• Molecular Weight: 87,747 Da
• NCBI Official Full Name: progesterone receptor isoform B
• NCBI Official Synonym Full Names: progesterone receptor
• NCBI Official Symbol: PGR
• NCBI Official Synonym Symbols: PR; NR3C3
• NCBI Protein Information: progesterone receptor; nuclear receptor subfamily 3 group C member 3
• UniProt Protein Name: Progesterone receptor
• Protein Family: Protein
• UniProt Gene Name: PGR
• UniProt Synonym Gene Names: NR3C3; PR
• UniProt Entry Name: PRGR_HUMAN

Uniprot Description

PR: a nuclear hormone receptor and transcription factor. Regulates gene expression and affects cellular proliferation and differentiation in target tissues. Two splice-variant isoforms have been described.

Protein type: Nuclear receptor; DNA-binding

Chromosomal Location of Human Ortholog: 11q22-q23

Cellular Component: nucleoplasm; mitochondrial outer membrane

Molecular Function: protein binding; ligand-dependent nuclear receptor activity; enzyme binding; DNA binding; zinc ion binding; steroid hormone receptor activity; steroid binding; receptor binding

Biological Process: transcription initiation from RNA polymerase II promoter; progesterone receptor signaling pathway; cell-cell signaling; epithelial cell maturation; positive regulation of transcription from RNA polymerase II promoter; steroid hormone mediated signaling; gene expression; signal transduction; ovulation from ovarian follicle; regulation of epithelial cell proliferation

Disease: Progesterone Resistance