Western Blot (WB)
((0.3ug/ml) staining of Human Frontal Cortex (A), Mouse (B) and Rat (C) Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat ELMO1 Polyclonal Antibody | anti-ELMO1 antibody
Goat anti-ELMO1 Antibody
Western Blot: Approx 83kDa band observed in Human Brain (Frontal Cortex) lysates, in Mouse and Rat Brain lysates and in lysates of cell line Jurkat (calculated MW of 83.8kDa according to NP_055615.8). Recommended concentration: 0.1-0.3ug/ml.
Immunoprecipitation: This antibody has been successfully used in IP from BAEC cells as described in the following paper: Epting et al, Circ Res.2010 Jul 9;107(1): 45-55; PMID: 20466982. IP; An anonymous customer found positive results in IP on Human promyelocytic leukemia cells (HL60).
Western Blot (WB)
((0.3ug/ml) staining of Human Frontal Cortex (A), Mouse (B) and Rat (C) Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0.3ug/ml) staining of Human Frontal Cortex (A), Mouse (B) and Rat (C) Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0.3ug/ml) staining of Jurkat cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0.3ug/ml) staining of Jurkat cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the engulfment and cell motility protein family. These proteins interact with dedicator of cytokinesis proteins to promote phagocytosis and cell migration. Increased expression of this gene and dedicator of cytokinesis 1 may promote glioma cell invasion, and single nucleotide polymorphisms in this gene may be associated with diabetic nephropathy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]
Uniprot Description
ELMO1: Involved in cytoskeletal rearrangements required for phagocytosis of apoptotic cells and cell motility. Acts in assocation with DOCK1 and CRK. Was initially proposed to be required in complex with DOCK1 to activate Rac Rho small GTPases. May enhance the guanine nucleotide exchange factor (GEF) activity of DOCK1. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Cytoskeletal; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 7p14.1
Cellular Component: cytoplasm; cytosol; membrane; plasma membrane
Molecular Function: guanyl-nucleotide exchange factor activity; protein binding; SH3 domain binding
Biological Process: actin cytoskeleton organization and biogenesis; apoptosis; cell migration; cell motility; phagocytosis, engulfment; positive regulation of GTPase activity; Rac protein signal transduction; regulation of defense response to virus by virus; vascular endothelial growth factor receptor signaling pathway
Research Articles on ELMO1
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Product Notes
The ELMO1 elmo1 (Catalog #AAA420226) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-ELMO1 Antibody reacts with Tested: Human, Mouse, Rat, Cow, Zebrafish; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow, Zebrafish and may cross-react with other species as described in the data sheet. AAA Biotech's ELMO1 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB), Immunoprecipitation (IP). Peptide ELISA: Antibody detection limit dilution 1: 128000. Western Blot: Approx 83kDa band observed in Human Brain (Frontal Cortex) lysates, in Mouse and Rat Brain lysates and in lysates of cell line Jurkat (calculated MW of 83.8kDa according to NP_055615.8). Recommended concentration: 0.1-0.3ug/ml. Immunoprecipitation: This antibody has been successfully used in IP from BAEC cells as described in the following paper: Epting et al, Circ Res.2010 Jul 9;107(1): 45-55; PMID: 20466982. IP; An anonymous customer found positive results in IP on Human promyelocytic leukemia cells (HL60). Researchers should empirically determine the suitability of the ELMO1 elmo1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: PKEPSNYDFV YDCN. It is sometimes possible for the material contained within the vial of "ELMO1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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