Rabbit Elk1 Polyclonal Antibody | anti-Elk1 antibody

Elk1 (S383) Polyclonal Antibody

Cat. Num. AAA3000485

• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Immunoprecipitation
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: Elk1; Polyclonal Antibody; Elk1 (S383) Polyclonal Antibody; ELK 1; ELK1 member of ETS oncogene family; ELK1 protein; ELK1; ELK2 member of ETS oncogene family; ETS domain containing protein Elk 1; ETS domain containing protein Elk1; ETS domain protein Elk1; ETS domain-containing protein Elk-1; ETS like gene 1; Member of ETS oncogene family; Oncogene Elk1; Tyrosine kinase (ELK1) oncogene; anti-Elk1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Elk1 (S383) polyclonal antibody detects endogenous levels of Elk1 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 428

• Applicable Applications for anti-Elk1 antibody: Immunohistochemistry (IHC), Immunoprecipitation (IP)
• Application Notes: IHC: 1:50-1:200; IP: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 350-400 of Human Elk1.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of Elk1 (S383) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.)

Related Product Information for anti-Elk1 antibody

Ets-1 is the prototype member of a family of genes identified on the basis of homology to the v-Ets oncogene isolated from the E26 erythroblastosis virus. This family of genes currently includes Ets-1, Ets-2, Erg-1-3, Elk-1, Elf-1, Elf-5, NERF, PU.1, PEA3, ERM, FEV, ER8l, Fli-1, TEL, Spi-B, ESE-1, ESE-3A, Net, ABT1 and ERF. Members of the Ets gene family exhibit varied patterns of tissue expression, and share a highly conserved carboxy-terminal domain containing a sequence related to the SV40 large T antigen nuclear localization signal sequence. This conserved domain is essential for Ets-1 binding to DNA and is likely to be responsible for the DNA binding activity of all members of the Ets gene family. Several of these proteins have been shown to recognize similar motifs in DNA that share a centrally located 5'-GGAA-3' element.

NCBI and Uniprot Product Information

• NCBI GI #: 166362732
• NCBI GeneID: 2002
• NCBI Accession #: NP_001107595.1
• NCBI GenBank Nucleotide #: NP_001107595.1
• UniProt Accession #: P19419
• Molecular Weight: ~ 45kDa
• NCBI Official Full Name: ETS domain-containing protein Elk-1 isoform a
• NCBI Official Synonym Full Names: ETS transcription factor ELK1
• NCBI Official Symbol: ELK1
• NCBI Protein Information: ETS domain-containing protein Elk-1
• UniProt Protein Name: ETS domain-containing protein Elk-1
• Protein Family: ETS domain-containing protein
• UniProt Gene Name: ELK1
• UniProt Entry Name: ELK1_HUMAN

NCBI Description

This gene is a member of the Ets family of transcription factors and of the ternary complex factor (TCF) subfamily. Proteins of the TCF subfamily form a ternary complex by binding to the the serum response factor and the serum response element in the promoter of the c-fos proto-oncogene. The protein encoded by this gene is a nuclear target for the ras-raf-MAPK signaling cascade. This gene produces multiple isoforms by using alternative translational start codons and by alternative splicing. Related pseudogenes have been identified on chromosomes 7 and 14. [provided by RefSeq, Mar 2012]

Uniprot Description

ELK1: a transcription factor of the Ets family and the ternary complex factor (TCF) subfamily. Forms a ternary complex by binding to the serum response factor and the serum response element in the promoter of the c-fos proto-oncogene. Mediates gene activity in response to serum and growth factors. Phosphorylated by MAP kinase pathways at a cluster of S/T motifs at its C-terminus; phosphorylation at these sites is critical for transcriptional activation by Elk-1. Two alternatively-spliced isoforms have been described.

Protein type: Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: Xp11.2

Cellular Component: nucleoplasm; mitochondrion; cell soma; dendrite; nerve terminal; nucleus

Molecular Function: protein binding; double-stranded DNA binding; chromatin binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; response to light stimulus; nerve growth factor receptor signaling pathway; MyD88-independent toll-like receptor signaling pathway; positive regulation of transcription, DNA-dependent; stress-activated MAPK cascade; toll-like receptor 3 signaling pathway; toll-like receptor 2 signaling pathway; toll-like receptor 10 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; toll-like receptor 5 signaling pathway; toll-like receptor signaling pathway; innate immune response; positive regulation of transcription from RNA polymerase II promoter; toll-like receptor 9 signaling pathway; cell differentiation; toll-like receptor 4 signaling pathway