Rabbit ELK1 Polyclonal Antibody | anti-ELK1 antibody

ELK1, Phosphorylated (S383) (ELK1, ETS-Domain Protein Elk-1) (Biotin)

Cat. Num. AAA6415729

• Reactivity: Human, Mouse, Rat
• Applications: Immunofluorescence, Western Blot, Immunohistochemistry
• Purity: Purified by affinity chromatography.
• Synonyms: ELK1; Polyclonal Antibody; Phosphorylated (S383) (ELK1; ETS-Domain Protein Elk-1) (Biotin); anti-ELK1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes ELK1, phosphorylated S383. Species Crossreactivity: human, mouse, rat
• Purity/Purification: Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
• Concentration: ~1mg/ml (varies by lot)

• Applicable Applications for anti-ELK1 antibody: Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: IF: 1:50-1:100; WB: 1:500-1:1000; IHC: 1:50-1:100; Applications are based on unconjugated antibody.
• Immunogen: Phospho specific peptide corresponding to residues surrounding S383 of human ELK1.
• Conjugate: Biotin
• Antigen Modification: Phosphorylated
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of extracts from HT29 cells untreated or treated with UV, using Phospho-ELK1-S383 antibody.)

Immunofluorescence (IF)

(Immunofluorescence analysis of MCF7 cell using Phospho-ELK1-S383 antibody. Blue: DAPI for nuclear staining.)

Product Categories/Family for anti-ELK1 antibody

Antibodies; Transcription Factors

NCBI and Uniprot Product Information

• NCBI GI #: 166362732
• NCBI GeneID: 2002
• NCBI Accession #: NP_001107595.1
• NCBI GenBank Nucleotide #: NM_001114123.2
• UniProt Accession #: P19419; O75606; O95058; Q969X8; Q9UJM4; B2R7H4
• Molecular Weight: 44,888 Da
• NCBI Official Full Name: ETS domain-containing protein Elk-1 isoform a
• NCBI Official Synonym Full Names: ELK1, member of ETS oncogene family
• NCBI Official Symbol: ELK1
• NCBI Protein Information: ETS domain-containing protein Elk-1; ETS-like gene 1; tyrosine kinase (ELK1) oncogene
• UniProt Protein Name: ETS domain-containing protein Elk-1
• Protein Family: ETS domain-containing protein
• UniProt Gene Name: ELK1
• UniProt Entry Name: ELK1_HUMAN

NCBI Description

This gene is a member of the Ets family of transcription factors and of the ternary complex factor (TCF) subfamily. Proteins of the TCF subfamily form a ternary complex by binding to the the serum response factor and the serum response element in the promoter of the c-fos proto-oncogene. The protein encoded by this gene is a nuclear target for the ras-raf-MAPK signaling cascade. This gene produces multiple isoforms by using alternative translational start codons and by alternative splicing. Related pseudogenes have been identified on chromosomes 7 and 14. [provided by RefSeq, Mar 2012]

Uniprot Description

ELK1: a transcription factor of the Ets family and the ternary complex factor (TCF) subfamily. Forms a ternary complex by binding to the serum response factor and the serum response element in the promoter of the c-fos proto-oncogene. Mediates gene activity in response to serum and growth factors. Phosphorylated by MAP kinase pathways at a cluster of S/T motifs at its C-terminus; phosphorylation at these sites is critical for transcriptional activation by Elk-1. Two alternatively-spliced isoforms have been described.

Protein type: Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: Xp11.2

Cellular Component: nucleoplasm; mitochondrion; cell soma; dendrite; nerve terminal; nucleus

Molecular Function: protein binding; double-stranded DNA binding; chromatin binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; response to light stimulus; nerve growth factor receptor signaling pathway; MyD88-independent toll-like receptor signaling pathway; positive regulation of transcription, DNA-dependent; stress-activated MAPK cascade; toll-like receptor 3 signaling pathway; toll-like receptor 2 signaling pathway; toll-like receptor 10 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; toll-like receptor 5 signaling pathway; toll-like receptor signaling pathway; innate immune response; positive regulation of transcription from RNA polymerase II promoter; toll-like receptor 9 signaling pathway; cell differentiation; toll-like receptor 4 signaling pathway