Rabbit anti-Human Prostate Specific Antigen Polyclonal Antibody | anti-PSA antibody

Prostate Specific Antigen, CT (PSA, APS, Kallikrein-3, KLK3, hK3, Kallikrein III, Kallikrein-related Peptidase 3, KLK2A1, P-30 Antigen, Seminin, Semenogelase, gamma-Seminoprotein) (MaxLight 550)

Cat. Num. AAA6494817

• Gene Names: KLK3; APS; PSA; hK3; KLK2A1
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: Prostate Specific Antigen; Polyclonal Antibody; CT (PSA; APS; Kallikrein-3; KLK3; hK3; Kallikrein III; Kallikrein-related Peptidase 3; KLK2A1; P-30 Antigen; Seminin; Semenogelase; gamma-Seminoprotein) (MaxLight 550); EC=3.4.21.77; Gamma-Seminoprotein; anti-PSA antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human KLK3.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight550.

• Applicable Applications for anti-PSA antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-1:500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human KLK3 (KLH).
• Conjugate: MaxLight550
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-KLK3 Pab in mouse brain tissue lysate.KLK3(arrow) was detected using the purified Pab.)

Product Categories/Family for anti-PSA antibody

Antibodies; Cancer Markers

References

1. Binnie, M.C., et al., Prostate 63(4):309-315 (2005). 2. Habib, F.K., et al., Int. J. Cancer 114(2):190-194 (2005). 3. Laidler, P., et al., Arch. Biochem. Biophys. 435(1):1-14 (2005). 4. Dallas, S.L., et al., J. Cell. Physiol. 202(2):361-370 (2005). 5. Olsson, A.Y., et al., Genomics 84(1):147-156 (2004).

NCBI and Uniprot Product Information

• NCBI GI #: 4502173
• NCBI GeneID: 354
• NCBI Accession #: NP_001639
• NCBI GenBank Nucleotide #: NM_001648.2
• UniProt Accession #: P07288; Q15096; Q16272; Q86TG8; Q8IXI4; C9JXH3; G3V0H4; G3XAE3
• Molecular Weight: 28.8 kDa (262aa)
• NCBI Official Full Name: prostate-specific antigen isoform 1 preproprotein
• NCBI Official Synonym Full Names: kallikrein related peptidase 3
• NCBI Official Symbol: KLK3
• NCBI Official Synonym Symbols: APS; PSA; hK3; KLK2A1
• NCBI Protein Information: prostate-specific antigen
• UniProt Protein Name: Prostate-specific antigen
• UniProt Gene Name: KLK3
• UniProt Synonym Gene Names: APS; PSA; Seminin
• UniProt Entry Name: KLK3_HUMAN

NCBI Description

Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Its protein product is a protease present in seminal plasma. It is thought to function normally in the liquefaction of seminal coagulum, presumably by hydrolysis of the high molecular mass seminal vesicle protein. Serum level of this protein, called PSA in the clinical setting, is useful in the diagnosis and monitoring of prostatic carcinoma. Alternate splicing of this gene generates several transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

PSA: Hydrolyzes semenogelin-1 thus leading to the liquefaction of the seminal coagulum. Forms heterodimer with SERPINA5. Inhibited by SERPINA5. Activity is strongly inhibited by Zn2+, 100 times more abundant in semen than in serum. This inhibition is relieved by exposure to semenogelins, which are avid zinc binders. Belongs to the peptidase S1 family. Kallikrein subfamily.

Protein type: Secreted; Protease; EC 3.4.21.77; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 19q13.41

Cellular Component: extracellular region; nucleus

Molecular Function: protein binding; serine-type peptidase activity; serine-type endopeptidase activity

Biological Process: negative regulation of angiogenesis; cellular protein metabolic process; proteolysis