Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Rabbit anti-Rat Elastase 3B (ELA3B) Polyclonal Antibody | anti-ELA3B antibody
Polyclonal Antibody to Elastase 3B (ELA3B)
Gene Names
Cela3b; Ela3b
Reactivity
Rat
Applications
Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
Purity
Affinity Chromatography
Synonyms
Elastase 3B (ELA3B); Polyclonal Antibody; Polyclonal Antibody to Elastase 3B (ELA3B); anti-ELA3B antibody
Host
Rabbit
Reactivity
Rat
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against ELA3B. It has been selected for its ability to recognize ELA3B in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
500ug/mL (varies by lot)
Sequence
Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below.
MGHHHHHHSGSEF-VVN GEDAVPYSWP WQVSLQYEKD GSFHHTCGGT LIAPDWVMTA GHCISTSRTY QVVLGEFERG VEEGPEQVIP VNAGDLFVHP KWNSNCVSCG NDIALVKLSR SAQLGDTVQL ACLPPAGEIL PNGAPCYISG WGRLSTNGPL PDKLQQALLP VVDYAHCSKW DWWGFSVKKT MVCAGGDIQS GCNGDSGGPL NCPAENGTWQ VHGVTSFVSS LGCNTLKKPT VFTRVSAFNE WIEETIANN
MGHHHHHHSGSEF-VVN GEDAVPYSWP WQVSLQYEKD GSFHHTCGGT LIAPDWVMTA GHCISTSRTY QVVLGEFERG VEEGPEQVIP VNAGDLFVHP KWNSNCVSCG NDIALVKLSR SAQLGDTVQL ACLPPAGEIL PNGAPCYISG WGRLSTNGPL PDKLQQALLP VVDYAHCSKW DWWGFSVKKT MVCAGGDIQS GCNGDSGGPL NCPAENGTWQ VHGVTSFVSS LGCNTLKKPT VFTRVSAFNE WIEETIANN
Sequence Length
269
Applicable Applications for anti-ELA3B antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunogen
Recombinant ELA3B (Val28~Asn269) expressed in E.coli.
Cross Reactivity
Rat
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2074161
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DABstainingonIHC-P.Samples:RatTissue))
Immunohistochemistry (IHC)
(DABstainingonIHC-P.Samples:RatTissue))
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
28,916 Da
NCBI Official Full Name
chymotrypsin-like elastase family member 3B
NCBI Official Synonym Full Names
chymotrypsin-like elastase family, member 3B
NCBI Official Symbol
Cela3b
NCBI Official Synonym Symbols
Ela3b
NCBI Protein Information
chymotrypsin-like elastase family member 3B
UniProt Protein Name
Chymotrypsin-like elastase family, member 3B
UniProt Gene Name
Cela3b
Uniprot Description
CELA3B: Efficient protease with alanine specificity but only little elastolytic activity. Belongs to the peptidase S1 family. Elastase subfamily.
Protein type: EC 3.4.21.70; Protease
Chromosomal Location of Human Ortholog: 5q36
Cellular Component: extracellular space
Molecular Function: serine-type endopeptidase activity
Biological Process: cholesterol metabolic process; proteolysis
Similar Products
Product Notes
The ELA3B cela3b (Catalog #AAA2006917) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Elastase 3B (ELA3B) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Elastase 3B (ELA3B) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the ELA3B cela3b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below. MGHHHHHHSG SEF-VVN GEDAVPYSWP WQVSLQYEKD GSFHHTCGGT LIAPDWVMTA GHCISTSRTY QVVLGEFERG VEEGPEQVIP VNAGDLFVHP KWNSNCVSCG NDIALVKLSR SAQLGDTVQL ACLPPAGEIL PNGAPCYISG WGRLSTNGPL PDKLQQALLP VVDYAHCSKW DWWGFSVKKT MVCAGGDIQS GCNGDSGGPL NCPAENGTWQ VHGVTSFVSS LGCNTLKKPT VFTRVSAFNE WIEETIANN. It is sometimes possible for the material contained within the vial of "Elastase 3B (ELA3B), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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