Western Blot (WB)
(Western Blot analysis of LOVO cells using EKLF Polyclonal Antibody.)
Rabbit anti-Human EKLF Polyclonal Antibody | anti-EKLF antibody
EKLF Polyclonal Antibody
ELISA: 1:20000
Not yet tested in other applications.
Western Blot (WB)
(Western Blot analysis of LOVO cells using EKLF Polyclonal Antibody.)
Western Blot (WB)
(Western Blot analysis of LOVO cells using EKLF Polyclonal Antibody.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a hematopoietic-specific transcription factor that induces high-level expression of adult beta-globin and other erythroid genes. The zinc-finger protein binds to the DNA sequence CCACACCCT found in the beta hemoglobin promoter. Heterozygous loss-of-function mutations in this gene result in the dominant In(Lu) blood phenotype. [provided by RefSeq, Oct 2009]
Uniprot Description
KLF1: Transcription regulator of erythrocyte development that probably serves as a general switch factor during erythropoiesis. Is a dual regulator of fetal-to-adult globin switching. Binds to the CACCC box in the beta-globin gene promoter and acts as a preferential activator of this gene. Furthermore, it binds to the BCL11A promoter and activates expression of BCL11A, which in turn represses the HBG1 and HBG2 genes. This dual activity ensures that, in most adults, fetal hemoglobin levels are low. Able to activate CD44 and AQP1 promoters. When sumoylated, acts as a transcriptional repressor by promoting interaction with CDH2/MI2beta and also represses megakaryocytic differentiation. Defects in KLF1 are the cause of congenital dyserythropoietic anemia type 4 (CDA4). It is a blood disorder characterized by ineffective erythropoiesis and hemolysis resulting in anemia. Circulating erythroblasts and erythroblasts in the bone marrow show various morphologic abnormalities. Affected individuals with CDA4 also have increased levels of fetal hemoglobin. Belongs to the krueppel C2H2-type zinc-finger protein family.
Protein type: DNA-binding; C2H2-type zinc finger protein; Transcription factor
Chromosomal Location of Human Ortholog: 19p13.2
Cellular Component: nuclear chromatin; nucleus
Molecular Function: metal ion binding; transcription factor activity
Biological Process: chromatin remodeling; erythrocyte maturation; transcription, DNA-dependent; embryonic hemopoiesis; in utero embryonic development; positive regulation of transcription, DNA-dependent; erythrocyte differentiation; liver development
Disease: Fetal Hemoglobin Quantitative Trait Locus 6; Anemia, Congenital Dyserythropoietic, Type Iv; Blood Group--lutheran Inhibitor
Research Articles on EKLF
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Product Notes
The EKLF klf1 (Catalog #AAA9410799) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The EKLF Polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's EKLF can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). Western Blot: 1:500 - 1:2000 ELISA: 1:20000 Not yet tested in other applications. Researchers should empirically determine the suitability of the EKLF klf1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EKLF, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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