Western Blot (WB)
(Western blot analysis of eIF5A expression in Jurkat cell lysate.)
Rabbit eIF5A Polyclonal Antibody | anti-eIF5A antibody
Anti-eIF5A Rabbit pAb
Gene Names
EIF5A; EIF-5A; EIF5A1; eIF5AI
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Flow Cytometry, Functional Assay
Purity
Affinity Chromatography
Synonyms
eIF5A; Polyclonal Antibody; Anti-eIF5A Rabbit pAb; EIF-5A; EIF5A1; eIF5AI; Eukaryotic initiation factor 5A; anti-eIF5A antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
eIF5A Antibody detects endogenous levels of eIF5A
Purity/Purification
Affinity Chromatography
Form/Format
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Sequence Length
184
Applicable Applications for anti-eIF5A antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:5000-1:10000
IHC: 1:50-1:200
ICC/IF: 1:100-1:500
IP: 1:50
FC/FACS: 1:50
IHC: 1:50-1:200
ICC/IF: 1:100-1:500
IP: 1:50
FC/FACS: 1:50
Immunogen
A synthesized peptide derived from human eIF5A
Subcellular Location
Nucleus; Nuclear pore complex
Preparation and Storage
Store at 4 degree C for short term.
Store at-20 degree C for long term.
Avoid freeze/thaw cycle.
Store at-20 degree C for long term.
Avoid freeze/thaw cycle.
Western Blot (WB)
(Western blot analysis of eIF5A expression in Jurkat cell lysate.)
Western Blot (WB)
(Western blot analysis of eIF5A expression in Jurkat cell lysate.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human uterus cancer, using eIF5A Antibody.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human uterus cancer, using eIF5A Antibody.)
Immunofluorescence (IF)
(Immunofluorescent analysis of Hela cells, using eIF5A Antibody.)
Immunofluorescence (IF)
(Immunofluorescent analysis of Hela cells, using eIF5A Antibody.)
Related Product Information for anti-eIF5A antibody
mRNA-binding protein involved in translation elongation. Has an important function at the level of mRNA turnover, probably acting downstream of decapping. Involved in actin dynamics and cell cycle progression, mRNA decay and probably in a pathway involved in stress response and maintenance of cell wall integrity. With syntenin SDCBP, functions as a regulator of p53/TP53 and p53/TP53-dependent apoptosis. Regulates also TNF-alpha-mediated apoptosis.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
18kDa
NCBI Official Full Name
eukaryotic translation initiation factor 5A-1 isoform A
NCBI Official Synonym Full Names
eukaryotic translation initiation factor 5A
NCBI Official Symbol
EIF5A
NCBI Official Synonym Symbols
EIF-5A; EIF5A1; eIF5AI
NCBI Protein Information
eukaryotic translation initiation factor 5A-1
UniProt Protein Name
Eukaryotic translation initiation factor 5A-1
Protein Family
UniProt Gene Name
EIF5A
UniProt Synonym Gene Names
eIF-5A-1; eIF-5A1; eIF-5A
Uniprot Description
mRNA-binding protein involved in translation elongation. Has an important function at the level of mRNA turnover, probably acting downstream of decapping. Involved in actin dynamics and cell cycle progression, mRNA decay and probably in a pathway involved in stress response and maintenance of cell wall integrity. With syntenin SDCBP, functions as a regulator of p53/TP53 and p53/TP53-dependent apoptosis. Regulates also TNF-alpha-mediated apoptosis. Mediates effects of polyamines on neuronal process extension and survival. May play an important role in brain development and function, and in skeletal muscle stem cell differentiation. Also described as a cellular cofactor of human T-cell leukemia virus type I (HTLV-1) Rex protein and of human immunodeficiency virus type 1 (HIV-1) Rev protein, essential for mRNA export of retroviral transcripts.
Research Articles on eIF5A
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Product Notes
The eIF5A eif5a (Catalog #AAA4750248) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-eIF5A Rabbit pAb reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's eIF5A can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Flow Cytometry (FC/FACS). WB: 1:5000-1:10000 IHC: 1:50-1:200 ICC/IF: 1:100-1:500 IP: 1:50 FC/FACS: 1:50. Researchers should empirically determine the suitability of the eIF5A eif5a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "eIF5A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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