Rabbit anti-Human EIF4EBP1 Polyclonal Antibody | anti-EIF4EBP1 antibody

EIF4EBP1, ID (EIF4EBP1, Eukaryotic translation initiation factor 4E-binding protein 1, Phosphorylated heat-and acid-stable protein regulated by insulin 1) (MaxLight 650)

Cat. Num. AAA6295102

• Gene Names: EIF4EBP1; BP-1; 4EBP1; 4E-BP1; PHAS-I
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
• Purity: Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Synonyms: EIF4EBP1; Polyclonal Antibody; ID (EIF4EBP1; Eukaryotic translation initiation factor 4E-binding protein 1; Phosphorylated heat-and acid-stable protein regulated by insulin 1) (MaxLight 650); Phosphorylated heat-and acid-stable protein regulated by insulin 1; anti-EIF4EBP1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight650.

• Applicable Applications for anti-EIF4EBP1 antibody: Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; IHC: 1:10-50; WB: 1:50-100; Applications are based on unconjugated antibody.
• Immunogen: EIF4EBP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 38-69 amino acids from the Central region of human EIF4EBP1.
• Conjugate: MaxLight650
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of EIF4EBP1 (arrow) using rabbit polyclonal EIF4EBP1 Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the EIF4EBP1 gene.)

Immunohistochemistry (IHC)

(EIF4EBP1 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human breast tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of EIF4EBP1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(EIF4EBP1 Antibody (Center) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-EIF4EBP1 antibody

This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation. [provided by RefSeq].

Product Categories/Family for anti-EIF4EBP1 antibody

Antibodies; Insulin

References

She, Q.B., et al. Cancer Cell 18(1):39-51(2010). Aoyagi, M., et al. Proc. Natl. Acad. Sci. U.S.A. 107(6):2640-2645(2010). Naukkarinen, J., et al. PLoS Genet. 6 (6), E1000976 (2010): Kumar, A., et al. PLoS ONE 5 (1), E8730 (2010): Villalonga, P., et al. J. Biol. Chem. 284(51):35287-35296(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 4758258
• NCBI GeneID: 1978
• NCBI Accession #: NP_004086.1
• NCBI GenBank Nucleotide #: NM_004095.3
• UniProt Accession #: Q13541; Q6IBN3; B2R502; D3DSW8
• Molecular Weight: 12,580 Da
• NCBI Official Full Name: eukaryotic translation initiation factor 4E-binding protein 1
• NCBI Official Synonym Full Names: eukaryotic translation initiation factor 4E binding protein 1
• NCBI Official Symbol: EIF4EBP1
• NCBI Official Synonym Symbols: BP-1; 4EBP1; 4E-BP1; PHAS-I
• NCBI Protein Information: eukaryotic translation initiation factor 4E-binding protein 1; eIF4E-binding protein 1; phosphorylated heat- and acid-stable protein regulated by insulin 1
• UniProt Protein Name: Eukaryotic translation initiation factor 4E-binding protein 1
• Protein Family: Eukaryotic translation initiation factor 4E-binding protein
• UniProt Gene Name: EIF4EBP1
• UniProt Synonym Gene Names: 4E-BP1; eIF4E-binding protein 1; PHAS-I
• UniProt Entry Name: 4EBP1_HUMAN

NCBI Description

This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation. [provided by RefSeq, Jul 2008]

Uniprot Description

4E-BP1: binds to eIF4E, preventing its assembly into the EIF4F complex and inhibiting cap-dependent translation. Phosphorylation of 4E-BP1 disrupts this binding, activating cap-dependent translation. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the PI3 kinase pathway.

Protein type: Translation; Translation initiation

Chromosomal Location of Human Ortholog: 8p12

Cellular Component: nucleoplasm; protein complex; cytoplasm; cytosol

Molecular Function: protein binding; translation repressor activity; eukaryotic initiation factor 4E binding

Biological Process: negative regulation of translational initiation; response to ethanol; cellular protein metabolic process; TOR signaling pathway; translation; insulin receptor signaling pathway; translational initiation; gene expression; positive regulation of mitotic cell cycle; negative regulation of protein complex assembly; G1/S transition of mitotic cell cycle; lung development