Immunofluorescence (IF)
(Immunofluorescence staining of methanol-fixed MCF cells using eIF4E (Phospho-Ser209) antibody)
Rabbit eIF4E Polyclonal Antibody | anti-eIF4E antibody
eIF4E antibody
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Purified by immunoaffinity chromatography.
Synonyms
eIF4E; Polyclonal Antibody; eIF4E antibody; Rabbit polyclonal eIF4E antibody; Polyclonal eIF4E antibody; Anti-eIF4E antibody; eIFE 4 antibody; EIF4E1 antibody; Eukaryotic Translation Initiation Factor 4E antibody; Phospho Ser209 antibody; EIF4F antibody; MGC111573 antibody; eIFE 4; CBP antibody; eIFE-4 antibody; EIF4EL1 antibody; eIFE-4; anti-eIF4E antibody
Host
Rabbit
Clonality
Polyclonal
Purity/Purification
Purified by immunoaffinity chromatography.
Form/Format
Liquid form in PBS buffer (no MG2+ or Ca2+), pH 7.4, 150 mm NaCl,0.02% sodium azide and 50% glycerol.
Concentration
1 mg/ml (varies by lot)
Sequence Length
231
Applicable Applications for anti-eIF4E antibody
Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
IF: 1:100-1:200
IHC: 1:50-1:100
WB: 1:500-1:1000
IHC: 1:50-1:100
WB: 1:500-1:1000
Immunogen
eIF4E antibody was raised in Rabbit using the peptide sequence around phosphorylation site of serine 209 (S-G-S(p)-T-T) derived from Human eIF4E as the immunogen.
Cross-Reactivity
Human
Preparation and Storage
Store at 4 degree C for short term storage.
Aliquot and store at-20 degree C for long term storage.
Avoid repeated freeze/thaw cycles.
Aliquot and store at-20 degree C for long term storage.
Avoid repeated freeze/thaw cycles.
Immunofluorescence (IF)
(Immunofluorescence staining of methanol-fixed MCF cells using eIF4E (Phospho-Ser209) antibody)
Immunofluorescence (IF)
(Immunofluorescence staining of methanol-fixed MCF cells using eIF4E (Phospho-Ser209) antibody)
Western Blot (WB)
(Western Blot analysis of extracts from HT29 cells untreated or treated with Anisomycin using eIF4E (Phospho-Ser209) antibody)
Western Blot (WB)
(Western Blot analysis of extracts from HT29 cells untreated or treated with Anisomycin using eIF4E (Phospho-Ser209) antibody)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using eIF4E (Phospho-Ser209) antibody (left) or the same antibody preincubated with with blocking peptide (right))
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using eIF4E (Phospho-Ser209) antibody (left) or the same antibody preincubated with with blocking peptide (right))
Related Product Information for anti-eIF4E antibody
All eukaryotic cellular mRNAs are blocked at their 5-prime ends with the 7-methylguanosine cap structure, m7GpppX (where X is any nucleotide). This structure is involved in several cellular processes including enhanced translational efficiency, splicing, mRNA stability, and RNA nuclear export.
Product Categories/Family for anti-eIF4E antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
eIF4E
NCBI Official Symbol
eIF4E1
NCBI Protein Information
eIF4E
Protein Family
Research Articles on eIF4E
Similar Products
Product Notes
The eIF4E (Catalog #AAA5309586) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's eIF4E can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB). IF: 1:100-1:200 IHC: 1:50-1:100 WB: 1:500-1:1000. Researchers should empirically determine the suitability of the eIF4E for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "eIF4E, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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