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Western Blot (WB) (Fig-1: Western blot analysis of eIF4A2. Anti-eIF4A2 antibody (MBS668637) was used at 2 ug/ml on Jurkat lysate.)

Rabbit anti-Human eIF4A2 Polyclonal Antibody | anti-EIF4A2 antibody

Polyclonal Antibody to eIF4A2

Gene Names
EIF4A2; DDX2B; EIF4A; EIF4F; BM-010; eIF4A-II; eIF-4A-II
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
Purified
Protein A Chromatography
Synonyms
eIF4A2; Polyclonal Antibody; Polyclonal Antibody to eIF4A2; Eukaryotic initiation factor 4A-II; DDX2B; EIF4F; eIF4A-II; eIF-4A-II; ATP-dependent RNA helicase eIF4A-2; Antibody to eIF4A2; Anti-eIF4A2 Antibody; eIF4A2 Antibody; anti-EIF4A2 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified
Protein A Chromatography
Form/Format
25 ug in 50 ul/100 ug in 200 ul PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
Sequence Length
407
Applicable Applications for anti-EIF4A2 antibody
Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
WB: 1-2 ug/ml
IHC: 20 ug/ml
Immunogen Information
A partial length recombinant eIF4A2 protein (amino acids 1-250) was used as the immunogen for this antibody.
Antigen
eIF4A2
Preparation and Storage
Store the antibody at 4°C, stable for 6 months.
For long-term storage, store at -20°C.
Avoid repeated freeze and thaw cycles.

Western Blot (WB)

(Fig-1: Western blot analysis of eIF4A2. Anti-eIF4A2 antibody (MBS668637) was used at 2 ug/ml on Jurkat lysate.)

Western Blot (WB) (Fig-1: Western blot analysis of eIF4A2. Anti-eIF4A2 antibody (MBS668637) was used at 2 ug/ml on Jurkat lysate.)

Immunohistochemistry (IHC)

(Fig-2: Immunohistochemical analysis of eIF4A2. Anti- eIF4A2 antibody (11-8048) in human Spleen tissue at 20 ug/ml.)

Immunohistochemistry (IHC) (Fig-2: Immunohistochemical analysis of eIF4A2. Anti- eIF4A2 antibody (11-8048) in human Spleen tissue at 20 ug/ml.)

Immunohistochemistry (IHC)

(Fig-3: Immunohistochemical analysis of eIF4A2. Anti- eIF4A2 antibody (11-8048) in human Tonsil tissue at 20 ug/ml.)

Immunohistochemistry (IHC) (Fig-3: Immunohistochemical analysis of eIF4A2. Anti- eIF4A2 antibody (11-8048) in human Tonsil tissue at 20 ug/ml.)
Product Categories/Family for anti-EIF4A2 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
Eukaryotic initiation factor 4A-II
NCBI Official Synonym Full Names
eukaryotic translation initiation factor 4A2
NCBI Official Symbol
EIF4A2
NCBI Official Synonym Symbols
DDX2B; EIF4A; EIF4F; BM-010; eIF4A-II; eIF-4A-II
NCBI Protein Information
eukaryotic initiation factor 4A-II
UniProt Protein Name
Eukaryotic initiation factor 4A-II
UniProt Gene Name
EIF4A2
UniProt Synonym Gene Names
DDX2B; EIF4F; eIF-4A-II; eIF4A-II

Uniprot Description

EIF4A2: ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon. Belongs to the DEAD box helicase family. eIF4A subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.6.4.13; Helicase; RNA-binding; Translation; Translation initiation

Chromosomal Location of Human Ortholog: 3q27.3

Cellular Component: cytosol; eukaryotic translation initiation factor 4F complex; perinuclear region of cytoplasm

Molecular Function: ATP-dependent RNA helicase activity; ATPase activity; helicase activity; protein binding

Biological Process: poly(A) tail shortening; RNA secondary structure unwinding; translational initiation

Research Articles on EIF4A2

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Product Notes

The EIF4A2 eif4a2 (Catalog #AAA668637) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to eIF4A2 reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's eIF4A2 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), Western Blot (WB). WB: 1-2 ug/ml IHC: 20 ug/ml. Researchers should empirically determine the suitability of the EIF4A2 eif4a2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "eIF4A2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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