Western Blot (WB)
(WB: 1:1000Western blot analysis of EIF3S5 Antibody (N-term) in A2058 cell line lysates (35ug/lane). EIF3S5 (arrow) was detected using the purified Pab.)
Rabbit EIF3S5 Polyclonal Antibody | anti-EIF3S5 antibody
EIF3S5 Antibody (N-term) Purified Rabbit Polyclonal Antibody (Pab)
Predicted Reactivity: Monkey, Mouse
Predicted Reactivity: Monkey, Mouse
IHC-P: 1:50-100
FC/FACS: 1:10-50
Western Blot (WB)
(WB: 1:1000Western blot analysis of EIF3S5 Antibody (N-term) in A2058 cell line lysates (35ug/lane). EIF3S5 (arrow) was detected using the purified Pab.)
Western Blot (WB)
(WB: 1:1000Western blot analysis of EIF3S5 Antibody (N-term) in A2058 cell line lysates (35ug/lane). EIF3S5 (arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC)-Paraffin
(IHC-P: 1:50-100Formalin-fixed and paraffin-embedded human lung carcinoma reacted with EIF3S5 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)-Paraffin
(IHC-P: 1:50-100Formalin-fixed and paraffin-embedded human lung carcinoma reacted with EIF3S5 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS)
(FC: 1:10~50EIF3S5 Antibody (N-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(FC: 1:10~50EIF3S5 Antibody (N-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
NCBI and Uniprot Product Information
Uniprot Description
eIF3-epsilon: eukaryotic translation initiation factor 3, subunit 5 epsilon. Binds to the 40s ribosome and promotes the binding of methionyl-tRNAi and mRNA. Associates with the complex p170-eIF3. eIF-3 is composed of at least 12 different subunits.
Protein type: Translation; Translation initiation; EC 3.4.19.12
Chromosomal Location of Human Ortholog: 11p15.4
Cellular Component: eukaryotic translation initiation factor 3 complex; membrane; cytosol
Molecular Function: protein binding; translation initiation factor binding; translation initiation factor activity; ubiquitin-specific protease activity
Biological Process: protein deubiquitination; cellular protein metabolic process; translation; translational initiation; gene expression; proteolysis; formation of translation preinitiation complex; regulation of translational initiation
Research Articles on EIF3S5
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Product Notes
The EIF3S5 eif3f (Catalog #AAA9232378) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The EIF3S5 Antibody (N-term) Purified Rabbit Polyclonal Antibody (Pab) reacts with Human Predicted Reactivity: Monkey, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's EIF3S5 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Flow Cytometry (FC/FACS), Immunohistochemistry-Paraffin (IHC-P), ELISA (EIA). WB: 1:1000 IHC-P: 1:50-100 FC/FACS: 1:10-50. Researchers should empirically determine the suitability of the EIF3S5 eif3f for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EIF3S5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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