Rabbit anti-Human EIF2S3L Polyclonal Antibody | anti-EIF2S3L antibody

EIF2S3L, ID (EIF2S3L, Putative eukaryotic translation initiation factor 2 subunit 3-like protein, Eukaryotic translation initiation factor 2 subunit gamma A) (FITC)

Cat. Num. AAA6294965

• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: EIF2S3L; Polyclonal Antibody; ID (EIF2S3L; Putative eukaryotic translation initiation factor 2 subunit 3-like protein; Eukaryotic translation initiation factor 2 subunit gamma A) (FITC); Eukaryotic translation initiation factor 2 subunit gamma A; anti-EIF2S3L antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-EIF2S3L antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: EIF2S3L antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 273-299 amino acids from the Central region of human EIF2S3L.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(EIF2S3L Antibody (Center) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the EIF2S3L antibody detected the EIF2S3L protein (arrow).)

Related Product Information for anti-EIF2S3L antibody

eIF-2 functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B (By similarity).

Product Categories/Family for anti-EIF2S3L antibody

Antibodies; Translation Initiation Factors

NCBI and Uniprot Product Information

• NCBI GI #: 205830883
• UniProt Accession #: Q2VIR3; Q5I0X0; Q6KF84
• Molecular Weight: 50,544 Da
• NCBI Official Full Name: Putative eukaryotic translation initiation factor 2 subunit 3-like protein
• UniProt Protein Name: Putative eukaryotic translation initiation factor 2 subunit 3-like protein
• Protein Family: Putative eukaryotic translation initiation factor 2 subunit 3-like protein
• UniProt Gene Name: EIF2S3L
• UniProt Synonym Gene Names: eIF-2gA

Uniprot Description

As a subunit of eukaryotic initiation factor 2 (eIF2), involved in the early steps of protein synthesis. In the presence of GTP, eIF2 forms a ternary complex with initiator tRNA Met-tRNAi and then recruits the 40S ribosomal complex, a step that determines the rate of protein translation. This step is followed by mRNA binding to form the 43S pre-initiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF2 and release of an eIF2-GDP binary complex. In order for eIF2 to recycle and catalyze another round of initiation, the GDP bound to eIF2 must exchange with GTP by way of a reaction catalyzed by eIF2B (). Along with its paralog on chromosome Y, may contribute to spermatogenesis up to the round spermatid stage ().