Rabbit anti-Human EIF2C2 Polyclonal Antibody | anti-EIF2C2 antibody

EIF2C2, NT (Eif2c2, Ago2, Kiaa4215, Protein argonaute-2, Eukaryotic translation initiation factor 2C 2, Piwi/argonaute family protein meIF2C2, Protein slicer) (Azide free) (HRP)

Cat. Num. AAA6294922

• Gene Names: AGO2; Q10; EIF2C2
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, ELISA
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: EIF2C2; Polyclonal Antibody; NT (Eif2c2; Ago2; Kiaa4215; Protein argonaute-2; Eukaryotic translation initiation factor 2C 2; Piwi/argonaute family protein meIF2C2; Protein slicer) (Azide free) (HRP); Eif2c2; Protein slicer; anti-EIF2C2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human EIF2C2.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-EIF2C2 antibody: Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 27-59 in the N-terminal region of human EIF2C2, UniProt Accession #Q9UKV8. Species sequence homology: bovine, rabbit and rat
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in K562 cell line lysates (35ug/lane) usingMBS644624. This demonstrates that MBS644624 detected the EIF2C2 protein (arrow).)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue using MBS644624 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS644624 for immunohistochemistry.)

Testing Data

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Related Product Information for anti-EIF2C2 antibody

EIF2C2 is a member of the Argonaute family of proteins which play a role in RNA interference. The encoded protein is highly basic, and contains a PAZ domain and a PIWI domain. It may interact with dicer1 and play a role in short-interfering-RNA-mediated gene silencing.

Product Categories/Family for anti-EIF2C2 antibody

Antibodies; Translation Initiation Factors

References

1. Rivas, F.V., et al., Nat Struct Mol Biol 12(4):340-349 (2005). 2. Liu, J., et al., Science 305(5689):1437-1441 (2004). 3. Nelson, P.T., et al., RNA 10(3):387-394 (2004). 4. Sasaki, T., et al., Genomics 82(3):323-330 (2003). 5. Doi, N., et al., Curr. Biol. 13(1):41-46 (2003).

NCBI and Uniprot Product Information

• NCBI GI #: 229463006
• NCBI GeneID: 27161
• UniProt Accession #: Q9UKV8; Q8TCZ5; Q8WV58; Q96ID1
• Molecular Weight: 859
• NCBI Official Full Name: Protein argonaute-2
• NCBI Official Synonym Full Names: argonaute RISC catalytic component 2
• NCBI Official Symbol: AGO2
• NCBI Official Synonym Symbols: Q10; EIF2C2
• NCBI Protein Information: protein argonaute-2; PPD; hAgo2; eIF2C 2; eIF-2C 2; argonaute2; argonaute 2; protein slicer; PAZ Piwi domain protein; eukaryotic translation initiation factor 2C, 2
• UniProt Protein Name: Protein argonaute-2
• UniProt Gene Name: AGO2
• UniProt Synonym Gene Names: hAgo2
• UniProt Entry Name: AGO2_HUMAN

NCBI Description

This gene encodes a member of the Argonaute family of proteins which play a role in RNA interference. The encoded protein is highly basic, and contains a PAZ domain and a PIWI domain. It may interact with dicer1 and play a role in short-interfering-RNA-mediated gene silencing. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009]

Uniprot Description

AGO2: the endonuclease in RNA-induced silencing complexes (RISC) that cleaves siRNA/mRNA heteroduplexes bound to RISC. Ago2, along with Dicer and TRBP, are major components of RISC. Interacts with Dicer1 through its Piwi domain. Required for proper fibroblast growth factor signaling during gastrulation. Essential for embryonic development as well as RNA-mediated gene silencing (RNAi).

Protein type: RNA processing; RNA-binding; EC 3.1.26.n2

Chromosomal Location of Human Ortholog: 8q24

Cellular Component: nucleoplasm; polysome; mRNA cap complex; membrane; cytoplasm; ribonucleoprotein complex; cytosol

Molecular Function: mRNA binding; endoribonuclease activity; protein binding; miRNA binding; siRNA binding; translation initiation factor activity; metal ion binding; RNA 7-methylguanosine cap binding

Biological Process: epidermal growth factor receptor signaling pathway; negative regulation of translational initiation; Notch signaling pathway; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; nerve growth factor receptor signaling pathway; transcription, DNA-dependent; RNA interference, siRNA loading onto RISC; translation; miRNA-mediated gene silencing, mRNA cleavage; miRNA-mediated gene silencing, miRNA loading onto RISC; miRNA-mediated gene silencing, negative regulation of translation; post-embryonic development; regulation of transcription, DNA-dependent; pre-microRNA processing; translational initiation; innate immune response; RNA-mediated gene silencing; gene expression; posttranscriptional gene silencing