Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using AGO2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 20s.)
Rabbit anti-Human, Mouse EIF2C2 Polyclonal Antibody | anti-AGO2 antibody
EIF2C2 Polyclonal Antibody
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using AGO2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 20s.)
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using AGO2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 20s.)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded mouse brain using AGO2 antibody at dilution of 1:100 (40x lens).)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded mouse brain using AGO2 antibody at dilution of 1:100 (40x lens).)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human stomach using AGO2 antibody at dilution of 1:100 (40x lens).)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human stomach using AGO2 antibody at dilution of 1:100 (40x lens).)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human stomach using AGO2 antibody at dilution of 1:100 (40x lens).)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human stomach using AGO2 antibody at dilution of 1:100 (40x lens).)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded mouse brain using AGO2 antibody at dilution of 1:100 (40x lens).)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded mouse brain using AGO2 antibody at dilution of 1:100 (40x lens).)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the Argonaute family of proteins which play a role in RNA interference. The encoded protein is highly basic, and contains a PAZ domain and a PIWI domain. It may interact with dicer1 and play a role in short-interfering-RNA-mediated gene silencing. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009]
Uniprot Description
AGO2: the endonuclease in RNA-induced silencing complexes (RISC) that cleaves siRNA/mRNA heteroduplexes bound to RISC. Ago2, along with Dicer and TRBP, are major components of RISC. Interacts with Dicer1 through its Piwi domain. Required for proper fibroblast growth factor signaling during gastrulation. Essential for embryonic development as well as RNA-mediated gene silencing (RNAi).
Protein type: RNA processing; RNA-binding; EC 3.1.26.n2
Chromosomal Location of Human Ortholog: 8q24
Cellular Component: nucleoplasm; polysome; mRNA cap complex; membrane; cytoplasm; ribonucleoprotein complex; cytosol
Molecular Function: mRNA binding; endoribonuclease activity; protein binding; miRNA binding; siRNA binding; translation initiation factor activity; metal ion binding; RNA 7-methylguanosine cap binding
Biological Process: epidermal growth factor receptor signaling pathway; negative regulation of translational initiation; Notch signaling pathway; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; nerve growth factor receptor signaling pathway; transcription, DNA-dependent; RNA interference, siRNA loading onto RISC; translation; miRNA-mediated gene silencing, mRNA cleavage; miRNA-mediated gene silencing, miRNA loading onto RISC; miRNA-mediated gene silencing, negative regulation of translation; post-embryonic development; regulation of transcription, DNA-dependent; pre-microRNA processing; translational initiation; innate immune response; RNA-mediated gene silencing; gene expression; posttranscriptional gene silencing
Research Articles on AGO2
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Product Notes
The AGO2 ago2 (Catalog #AAA129814) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The EIF2C2 Polyclonal Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's EIF2C2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500 - 1:2000. Researchers should empirically determine the suitability of the AGO2 ago2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EIF2C2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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