Rabbit anti-Human EIF2AK3 Polyclonal Antibody | anti-EIF2AK3 antibody

EIF2AK3, NT (Q163) (Eukaryotic Translation Initiation Factor 2-alpha Kinase 3, PRKR-like Endoplasmic Reticulum Kinase, Pancreatic eIF2-alpha Kinase, HsPEK, PEK, PERK) (APC)

Cat. Num. AAA6475220

• Gene Names: EIF2AK3; PEK; WRS; PERK
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: EIF2AK3; Polyclonal Antibody; NT (Q163) (Eukaryotic Translation Initiation Factor 2-alpha Kinase 3; PRKR-like Endoplasmic Reticulum Kinase; Pancreatic eIF2-alpha Kinase; HsPEK; PEK; PERK) (APC); anti-EIF2AK3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PERK.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-EIF2AK3 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: ELISA: 1:1,000; WB: 1:50-1:100; IHC: 1:50-1:100; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the N-term region of human PERK (KLH).
• Conjugate: APC
• Note: Preservative Free
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of PERK Antibody (N-term Q163) in 293 cell line lysates (35ug/lane). PERK (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PERK antibody (N-term Q163), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Product Categories/Family for anti-EIF2AK3 antibody

Antibodies; Translation Initiation Factors

References

1. Delepine, M., et al., Nat. Genet. 25(4):406-409 (2000). 2. Shi, Y., et al., J. Biol. Chem. 274(9):5723-5730 (1999). 3. Sood, R., et al., Biochem. J. 346 Pt 2, 281-293 (2000).

NCBI and Uniprot Product Information

• NCBI GI #: 134304838
• NCBI GeneID: 9451
• NCBI Accession #: NP_004827
• NCBI GenBank Nucleotide #: NM_004836.6
• UniProt Accession #: Q9NZJ5; O95846; Q53QY0; Q53SB1; A0AVH1; A0AVH2; B2RCU9
• Molecular Weight: 125,216 Da
• NCBI Official Full Name: eukaryotic translation initiation factor 2-alpha kinase 3 isoform 1
• NCBI Official Synonym Full Names: eukaryotic translation initiation factor 2 alpha kinase 3
• NCBI Official Symbol: EIF2AK3
• NCBI Official Synonym Symbols: PEK; WRS; PERK
• NCBI Protein Information: eukaryotic translation initiation factor 2-alpha kinase 3
• UniProt Protein Name: Eukaryotic translation initiation factor 2-alpha kinase 3
• Protein Family: Eukaryotic translation initiation factor 2-alpha kinase
• UniProt Gene Name: EIF2AK3
• UniProt Synonym Gene Names: PEK; PERK; HsPEK
• UniProt Entry Name: E2AK3_HUMAN

NCBI Description

The protein encoded by this gene phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2, leading to its inactivation, and thus to a rapid reduction of translational initiation and repression of global protein synthesis. This protein is thought to modulate mitochondrial function. It is a type I membrane protein located in the endoplasmic reticulum (ER), where it is induced by ER stress caused by malfolded proteins. Mutations in this gene are associated with Wolcott-Rallison syndrome. [provided by RefSeq, Sep 2015]

Uniprot Description

PERK: a transmembrane protein kinase of the PEK family resident in the endoplasmic reticulum (ER) membrane and is linked to insulin processing. Couples ER stress to translation inhibition. Stress induces autophosphorylation of its kinase domain and increases its activity. Phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2 (eIF2alpha), leading to its inactivation and thus to a rapid reduction of translational initiation and repression of global protein synthesis. A critical effector of unfolded protein response (UPR)-induced G1 growth arrest due to the loss of cyclin d1. Forms dimers with BiP in resting cells. Oligomerizes in ER-stressed cells. LOF mutations cause Wolcott-Rallison syndrome (WRS), characterized by insulin-dependent diabetes in early infancy and, later, multiple system abnormalities. Neuronal death in Alzheimer?s and Parkinson?s diseases is thought to be due to ER stress and has been weakly linked to PEK.

Protein type: EC 2.7.11.1; Membrane protein, integral; Translation; Protein kinase, Other; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Other group; PEK family; PEK subfamily

Chromosomal Location of Human Ortholog: 2p12

Cellular Component: endoplasmic reticulum membrane; membrane; endoplasmic reticulum; cytoplasm; integral to endoplasmic reticulum membrane

Molecular Function: identical protein binding; protein serine/threonine kinase activity; protein binding; enzyme binding; protein phosphatase binding; ATP binding; eukaryotic translation initiation factor 2alpha kinase activity; protein kinase activity

Biological Process: fat cell differentiation; lactation; translation; positive regulation of protein binding; protein amino acid autophosphorylation; protein amino acid phosphorylation; bone mineralization; positive regulation of transcription from RNA polymerase I promoter; cellular response to glucose starvation; regulation of fatty acid metabolic process; angiogenesis; skeletal development; ER overload response; protein homooligomerization; caspase activation; endoplasmic reticulum organization and biogenesis; ossification; negative regulation of myelination; unfolded protein response; calcium-mediated signaling; chondrocyte development; SREBP-mediated signaling pathway; endocrine pancreas development; peptidyl-serine phosphorylation; unfolded protein response, activation of signaling protein activity; cellular protein metabolic process; insulin-like growth factor receptor signaling pathway; insulin secretion; negative regulation of translation; negative regulation of translation initiation in response to stress; negative regulation of apoptosis

Disease: Epiphyseal Dysplasia, Multiple, With Early-onset Diabetes Mellitus