Western Blot (WB)
(Western Blot analysis of EGR2 on MCF-7 and SH-SY-5Y cell lysates using anti-EGR2 at 1/500 dilution.)
Rabbit EGR2 Polyclonal Antibody | anti-EGR2 antibody
EGR2 Polyclonal Antibody
FC/FACS: 1:50-1:100
Aliquot store at -20 degree C.
Avoid repeated freeze/thaw cycles.
Western Blot (WB)
(Western Blot analysis of EGR2 on MCF-7 and SH-SY-5Y cell lysates using anti-EGR2 at 1/500 dilution.)
Western Blot (WB)
(Western Blot analysis of EGR2 on MCF-7 and SH-SY-5Y cell lysates using anti-EGR2 at 1/500 dilution.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of SH-SY-5Y cells with EGR2 antibody at 1/50 dilution (Fuchsia) compared with an unlabelled control (cells without incubation with primary antibody; Yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of SH-SY-5Y cells with EGR2 antibody at 1/50 dilution (Fuchsia) compared with an unlabelled control (cells without incubation with primary antibody; Yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a transcription factor with three tandem C2H2-type zinc fingers. Defects in this gene are associated with Charcot-Marie-Tooth disease type 1D (CMT1D), Charcot-Marie-Tooth disease type 4E (CMT4E), and with Dejerine-Sottas syndrome (DSS). Multiple transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Oct 2008]
Uniprot Description
EGR2: Sequence-specific DNA-binding transcription factor. Binds to two specific DNA sites located in the promoter region of HOXA4. Defects in EGR2 are a cause of congenital hypomyelination neuropathy (CHN). Inheritance can be autosomal dominant or recessive. Recessive CHN is also known as Charcot- Marie-Tooth disease type 4E (CMT4E). CHN is characterized clinically by early onset of hypotonia, areflexia, distal muscle weakness, and very slow nerve conduction velocities. Defects in EGR2 are a cause of Charcot-Marie-Tooth disease type 1D (CMT1D). CMT1D is a form of Charcot- Marie-Tooth disease, the most common inherited disorder of the peripheral nervous system. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathy or CMT1, and primary peripheral axonal neuropathy or CMT2. Neuropathies of the CMT1 group are characterized by severely reduced nerve conduction velocities (less than 38 m/sec), segmental demyelination and remyelination with onion bulb formations on nerve biopsy, slowly progressive distal muscle atrophy and weakness, absent deep tendon reflexes, and hollow feet. Defects in EGR2 are a cause of Dejerine-Sottas syndrome (DSS); also known as Dejerine-Sottas neuropathy (DSN) or hereditary motor and sensory neuropathy III (HMSN3). DSS is a severe degenerating neuropathy of the demyelinating Charcot-Marie- Tooth disease category, with onset by age 2 years. DSS is characterized by motor and sensory neuropathy with very slow nerve conduction velocities, increased cerebrospinal fluid protein concentrations, hypertrophic nerve changes, delayed age of walking as well as areflexia. There are both autosomal dominant and autosomal recessive forms of Dejerine-Sottas syndrome. Belongs to the EGR C2H2-type zinc-finger protein family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: DNA-binding; C2H2-type zinc finger protein; EC 6.3.2.-
Chromosomal Location of Human Ortholog: 10q21.1
Cellular Component: cytoplasm; nucleus
Molecular Function: protein binding; ubiquitin protein ligase binding; metal ion binding; chromatin binding; transcription factor activity; ligase activity
Biological Process: myelination; transcription from RNA polymerase II promoter; fat cell differentiation; facial nerve structural organization; rhombomere 5 formation; positive regulation of transcription, DNA-dependent; motor axon guidance; response to insulin stimulus; peripheral nervous system development; Schwann cell differentiation; rhythmic behavior; protein sumoylation; learning and/or memory; rhombomere 3 formation; positive regulation of transcription from RNA polymerase II promoter; brain development; protein export from nucleus; regulation of ossification; brain segmentation; regulation of neuronal synaptic plasticity; negative regulation of apoptosis
Disease: Neuropathy, Congenital Hypomyelinating Or Amyelinating, Autosomal Recessive; Hypertrophic Neuropathy Of Dejerine-sottas; Charcot-marie-tooth Disease, Demyelinating, Type 1d
Research Articles on EGR2
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Product Notes
The EGR2 egr2 (Catalog #AAA3012635) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The EGR2 Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's EGR2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Flow Cytometry (FC/FACS). WB: 1:500-1:1,000 FC/FACS: 1:50-1:100. Researchers should empirically determine the suitability of the EGR2 egr2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EGR2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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