Western Blot (WB)
(Western Blot analysis of Egr-2b in the zebrafish embryonic (24hpf) (A) and larva (6 dpf) (B) lysates with the MBS643174. An immunoreactive band at ~48kD was detected; the band was blocked by the immunizing peptide (C, D).)
Rabbit Egr-2b Polyclonal Antibody | anti-egr2b antibody
Egr-2b, ID (Early Growth Response 2b, egr2b, Dr.75080)
Gene Names
egr2b; egr2; cb427; krx20; KRX-20; krox20; zgc:92210; id:ibd5073; wu:fb71h10
Applications
ELISA, Western Blot
Purity
Affinity PurifiedPurified by immunoaffinity chromatography.
Synonyms
Egr-2b; Polyclonal Antibody; ID (Early Growth Response 2b; egr2b; Dr.75080); Anti -Egr-2b; anti-egr2b antibody
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes zebrafish Egr-2b.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a liquid in 40mM MOPS, pH 7.5, 0.1% BSA, 0.05% sodium azide, 50% glycerol.
Applicable Applications for anti-egr2b antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes
Suitable for use in ELISA and Western Blot.
Dilution: ELISA: 1:5000-1:20,000
Western Blot: 1:500-1:1000
Dilution: ELISA: 1:5000-1:20,000
Western Blot: 1:500-1:1000
Immunogen
Synthetic peptide corresponding to the intermediate region of zebrafish Egr-2b.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western Blot analysis of Egr-2b in the zebrafish embryonic (24hpf) (A) and larva (6 dpf) (B) lysates with the MBS643174. An immunoreactive band at ~48kD was detected; the band was blocked by the immunizing peptide (C, D).)
Western Blot (WB)
(Western Blot analysis of Egr-2b in the zebrafish embryonic (24hpf) (A) and larva (6 dpf) (B) lysates with the MBS643174. An immunoreactive band at ~48kD was detected; the band was blocked by the immunizing peptide (C, D).)
Related Product Information for anti-egr2b antibody
Early growth response 2b (egr2b) is also known as krox20. First detected during late gastrulation, found in the prospective neuroepithelium domain around 100% epiboly and begins to constrict along the neuroaxis by the end of gastrulation - around 10 hours post- fertilization (hpf). By 12hpf expression is restricted in two regions of the neuroepithelium of the prospective hindbrain; rhombomeres 3 and 5. Around 14hpf neural crest migration begins from the dorsal surface of R5 moving caudally into R6 and then ventrally towards the pharyngeal arches. Crest migration is not apparent at or after 16hpf and no migration is seen from R3. Expression is down-regulated first in R3 around 26hpf and later in R5 around 30hpf. Egr2b binds two specific DNA sites located in the promoter region of HOXA4.
Product Categories/Family for anti-egr2b antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
45,153 Da
NCBI Official Full Name
early growth response protein 2b
NCBI Official Synonym Full Names
early growth response 2b
NCBI Official Symbol
egr2b
NCBI Official Synonym Symbols
egr2; cb427; krx20; KRX-20; krox20; zgc:92210; id:ibd5073; wu:fb71h10
NCBI Protein Information
early growth response protein 2b; EGR-2b; krox-20; protein krx-20; zinc finger protein Krox-20
UniProt Protein Name
Early growth response protein 2b
Protein Family
UniProt Gene Name
egr2b
UniProt Synonym Gene Names
egr2; krx-20; krx20; EGR-2b
UniProt Entry Name
EGR2B_DANRE
Uniprot Description
Function: Sequence-specific DNA-binding transcription factor. Binds to two specific DNA sites located in the promoter region of HOXA4.
Subcellular location: Nucleus.
Developmental stage: First detected during late gastrulation, found in the prospective neuroepithelium domain around 100% epiboly and begins to constrict along the neuroaxis by the end of gastrulation - around 10 hours post-fertilization (hpf). By 12 hpf expression is restricted in two regions of the neuroepithelium of the prospective hindbrain; rhombomeres 3 and 5. Around 14 hpf neural crest migration begins from the dorsal surface of R5 moving caudally into R6 and then ventrally towards the pharyngeal arches. Crest migration is not apparent at or after 16 hpf and no migration is seen from R3. Expression is down-regulated first in R3 around 26 hpf and later in R5 around 30 hpf.
Sequence similarities: Belongs to the EGR C2H2-type zinc-finger protein family.Contains 3 C2H2-type zinc fingers.
Research Articles on egr2b
Similar Products
Product Notes
The egr2b egr2b (Catalog #AAA643174) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Egr-2b can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in ELISA and Western Blot. Dilution: ELISA: 1:5000-1:20,000 Western Blot: 1:500-1:1000. Researchers should empirically determine the suitability of the egr2b egr2b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Egr-2b, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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