Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Cyclin-D-binding Myb-like transcription factor 1 (DMTF1) Standard Curve.)
Rat Cyclin-D-Binding Myb-Like Transcription Factor 1 (DMTF1) ELISA Kit | DMTF1 elisa kit
Rat Cyclin-D-Binding Myb-Like Transcription Factor 1 (DMTF1) ELISA Kit
Reactivity
Rat
Synonyms
Cyclin-D-Binding Myb-Like Transcription Factor 1 (DMTF1); Rat Cyclin-D-Binding Myb-Like Transcription Factor 1 (DMTF1) ELISA Kit; DMTF1; DMP1; DMTF; FLJ25188; FLJ41265; FLJ76054; hDMP1; OTTHUMP00000205131; cyclin D-binding Myb-like protein 1; DMTF1 elisa kit
Reactivity
Rat
Specificity
Rat Cyclin-D-binding Myb-like transcription factor 1 (DMTF1) ELISA Kit has high sensitivity and excellent specificity for detection of Rat DMTF1. No significant cross-reactivity or interference between Rat DMTF1 and analogues was observed.
Sequence Length
690
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Cyclin-D-binding Myb-like transcription factor 1 (DMTF1) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Cyclin-D-binding Myb-like transcription factor 1 (DMTF1) Standard Curve.)
Related Product Information for DMTF1 elisa kit
Description: This Rat Cyclin-D-binding Myb-like transcription factor 1 (DMTF1) ELISA Kit employs a two-site sandwich ELISA to quantitate DMTF1 in samples. An antibody specific for DMTF1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any DMTF1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DMTF1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DMTF1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: DMTF1 encodes a transcription factor that contains a cyclin D-binding domain, three central Myb-like repeats, and two flanking acidic transactivation domains at the N- and C-termini. The encoded protein is induced by the oncogenic Ras signaling pathway and functions as a tumor suppressor by activating the transcription of ARF and thus the ARF-p53 pathway to arrest cell growth or induce apoptosis. It also activates the transcription of aminopeptidase N and may play a role in hematopoietic cell differentiation. The transcriptional activity of this protein is regulated by binding of D-cyclins. This gene is hemizygously deleted in approximately 40% of human non-small-cell lung cancer and is a potential prognostic and gene-therapy target for non-small-cell lung cancer.
Background: DMTF1 encodes a transcription factor that contains a cyclin D-binding domain, three central Myb-like repeats, and two flanking acidic transactivation domains at the N- and C-termini. The encoded protein is induced by the oncogenic Ras signaling pathway and functions as a tumor suppressor by activating the transcription of ARF and thus the ARF-p53 pathway to arrest cell growth or induce apoptosis. It also activates the transcription of aminopeptidase N and may play a role in hematopoietic cell differentiation. The transcriptional activity of this protein is regulated by binding of D-cyclins. This gene is hemizygously deleted in approximately 40% of human non-small-cell lung cancer and is a potential prognostic and gene-therapy target for non-small-cell lung cancer.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
cyclin-D-binding Myb-like transcription factor 1
NCBI Official Synonym Full Names
cyclin D binding myb-like transcription factor 1
NCBI Official Symbol
Dmtf1
NCBI Protein Information
cyclin-D-binding Myb-like transcription factor 1
UniProt Protein Name
Cyclin-D-binding Myb-like transcription factor 1
Protein Family
UniProt Gene Name
Dmtf1
UniProt Entry Name
DMTF1_RAT
NCBI Description
mouse homolog is a transcription factor that arrests the cell cycle in G1 phase [RGD, Feb 2006]
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Product Notes
The Rat DMTF1 dmtf1 (Catalog #AAA9712412) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9712412 ELISA Kit recognizes Rat DMTF1. It is sometimes possible for the material contained within the vial of "Cyclin-D-Binding Myb-Like Transcription Factor 1 (DMTF1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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