Rabbit anti-Human, Mouse EFTUD1 Polyclonal Antibody | anti-EFTUD1 antibody

EFTUD1, CT (EFTUD1, EFL1, FAM42A, Elongation factor Tu GTP-binding domain-containing protein 1, Elongation factor-like 1, Protein FAM42A) (Biotin)

Cat. Num. AAA6294623

• Gene Names: EFL1; RIA1; EFTUD1; FAM42A; HsT19294
• Reactivity: Human, Mouse
• Applications: Flow Cytometry, Functional Assay, ELISA, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: EFTUD1; Polyclonal Antibody; CT (EFTUD1; EFL1; FAM42A; Elongation factor Tu GTP-binding domain-containing protein 1; Elongation factor-like 1; Protein FAM42A) (Biotin); Protein FAM42A; anti-EFTUD1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-EFTUD1 antibody: Flow Cytometry (FC/FACS), ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; ELISA: 1:1,000; IHC: 1:50-100; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: EFTUD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1069-1098 amino acids from the C-terminal region of human EFTUD1.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(EFTUD1 Antibody (C-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the EFTUD1 antibody detected the EFTUD1 protein (arrow).)

Western Blot (WB)

(EFTUD1 Antibody (C-term) western blot analysis in mouse spleen tissue lysates (35ug/lane).This demonstrates the EFTUD1 antibody detected the EFTUD1 protein (arrow).)

Immunohistochemistry (IHC)

(EFTUD1 antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the EFTUD1 antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(EFTUD1 Antibody (C-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-EFTUD1 antibody

Antibodies; Binding Proteins

References

Nicolas, E., et al. Eur. J. Hum. Genet. 18(10):1107-1113(2010). Willer, C.J., et al. Nat. Genet. 41(1):25-34(2009). Harrington, J.J., et al. Nat. Biotechnol. 19(5):440-445(2001).

NCBI and Uniprot Product Information

• NCBI GI #: 94966754
• NCBI GeneID: 79631
• NCBI Accession #: NP_078856.4; NP_001035700.1
• NCBI GenBank Nucleotide #: NM_024580.5
• UniProt Accession #: Q7Z2Z2; Q9H8Z6; A6NKY5; B7Z6I0
• Molecular Weight: 119,887 Da
• NCBI Official Full Name: elongation factor-like GTPase 1 isoform 1
• NCBI Official Synonym Full Names: elongation factor like GTPase 1
• NCBI Official Symbol: EFL1
• NCBI Official Synonym Symbols: RIA1; EFTUD1; FAM42A; HsT19294
• NCBI Protein Information: elongation factor-like GTPase 1
• UniProt Protein Name: Elongation factor Tu GTP-binding domain-containing protein 1
• UniProt Gene Name: EFTUD1
• UniProt Synonym Gene Names: EFL1; FAM42A
• UniProt Entry Name: ETUD1_HUMAN