Mouse anti-Human CCL-21 Monoclonal Antibody | anti-CCL-21 antibody

Mouse Anti-Human CCL-21

Cat. Num. AAA690726

• Gene Names: CCL21; ECL; SLC; CKb9; TCA4; 6Ckine; SCYA21
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry
• Purity: Protein G chromatography
• Synonyms: CCL-21; Monoclonal Antibody; Mouse Anti-Human CCL-21; CCL21; ECL; SLC; CKb9; TCA4; 6Ckine; SCYA21; anti-CCL-21 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 2H26
• Purity/Purification: Protein G chromatography
• Form/Format: Lyophilized

• Applicable Applications for anti-CCL-21 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:1000; ICC: 1:100-1:200; ; Optimal dilutions should be determined by each laboratory for each application.
• Preparation: This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a mouse) immunized with human recombinant protein of CCL-21 (also called 6Ckine).
• Antigen: Recombinant human CCL-21
• Reconstitution Buffer: PBS (sterile)
• Reconstitution: Reconstitute the antibody with 200 ul sterile PBS and the final concentration is 500 ug/ml.
• Preparation and Storage: Lyophilized samples are stable for 2 years from date of receipt when stored at -70°C.; Reconstituted antibody can be aliquoted and stored frozen at < -20°C for at least for six months without detectable loss of activity.; Avoid repeated freeze and thaw cycles.

Immunohistochemistry (IHC)

Related Product Information for anti-CCL-21 antibody

6Ckine or CCL-21 is a novel CC chemokine discovered independently by three groups from the EST database. 6Ckine, also named SLC (Secondary Lymphoid-tissue Chemokine), CCL21 and Exodus2, shows 21 33% identity to other CC chemokines. 6Ckine contains the four conserved cysteines characteristic of beta chemokines plus two additional cysteines in its unusually long carboxy-lterminal domain. Human 6Ckine cDNA encodes a 134 amino acid residue, highly basic, precursor protein with a 23 amino acid residue signal peptide that is cleaved to form the predicted 111 amino acid residue mature protein. Mouse 6Ckine cDNA encodes a 133 amino acid residue protein with a 23 residue signal peptide that is cleaved to generate the 110 residue mature protein. Human and mouse 6Ckine are highly conserved, exhibiting 86% amino acid sequence identity. 6Ckine is constitutively expressed at high levels in lymphoid tissues such as lymph nodes, spleen and appendix. In mouse, high levels of 6Ckine mRNA are also detected in the lung. The gene for human 6Ckine has been localized at human chromosome 9p13 rather than chromosome 17 where the genes of many human CC chemokines are clustered. The 6Ckine gene location is within a region of about 100 kb from the gene for MIP3beta/ ELC, another novel CC chemokine.

NCBI and Uniprot Product Information

• NCBI GI #: 4506835
• NCBI GeneID: 6366
• NCBI Accession #: NP_002980.1
• NCBI GenBank Nucleotide #: NM_002989.3
• UniProt Accession #: O00585
• NCBI Official Full Name: C-C motif chemokine 21
• NCBI Official Synonym Full Names: chemokine (C-C motif) ligand 21
• NCBI Official Symbol: CCL21
• NCBI Official Synonym Symbols: ECL; SLC; CKb9; TCA4; 6Ckine; SCYA21
• NCBI Protein Information: C-C motif chemokine 21; beta chemokine exodus-2; secondary lymphoid tissue chemokine; Efficient Chemoattractant for Lymphocytes; small inducible cytokine subfamily A (Cys-Cys), member 21
• UniProt Protein Name: C-C motif chemokine 21
• UniProt Gene Name: CCL21
• UniProt Synonym Gene Names: SCYA21; SLC
• UniProt Entry Name: CCL21_HUMAN

NCBI Description

This gene is one of several CC cytokine genes clustered on the p-arm of chromosome 9. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. Similar to other chemokines the protein encoded by this gene inhibits hemopoiesis and stimulates chemotaxis. This protein is chemotactic in vitro for thymocytes and activated T cells, but not for B cells, macrophages, or neutrophils. The cytokine encoded by this gene may also play a role in mediating homing of lymphocytes to secondary lymphoid organs. It is a high affinity functional ligand for chemokine receptor 7 that is expressed on T and B lymphocytes and a known receptor for another member of the cytokine family (small inducible cytokine A19). [provided by RefSeq, May 2013]

Uniprot Description

Function: Inhibits hemopoiesis and stimulates chemotaxis. Chemotactic in vitro for thymocytes and activated T-cells, but not for B-cells, macrophages, or neutrophils. Shows preferential activity towards naive T-cells. May play a role in mediating homing of lymphocytes to secondary lymphoid organs. Binds to atypical chemokine receptor ACKR4 and mediates the recruitment of beta-arrestin (ARRB1/2) to ACKR4.

Subunit structure: Monomer. Binds to CCR7. Ref.8 Ref.9 Ref.10

Subcellular location: Secreted.

Tissue specificity: Highly expressed in high endothelial venules of lymph nodes, spleen and appendix. Intermediate levels found in small intestine, thyroid gland and trachea. Low level expression in thymus, bone marrow, liver, and pancreas. Also found in tonsil, fetal heart and fetal spleen.

Sequence similarities: Belongs to the intercrine beta (chemokine CC) family.