Rabbit anti-Human EFNB1 Polyclonal Antibody | anti-EFNB1 antibody

EFNB1, ID (EFNB1, EFL3, EPLG2, LERK2, Ephrin-B1, EFL-3, ELK ligand, EPH-related receptor tyrosine kinase ligand 2) (Azide free) (HRP)

Cat. Num. AAA6294592

• Gene Names: EFNB1; CFND; CFNS; EFL3; EPLG2; Elk-L; LERK2
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: EFNB1; Polyclonal Antibody; ID (EFNB1; EFL3; EPLG2; LERK2; Ephrin-B1; EFL-3; ELK ligand; EPH-related receptor tyrosine kinase ligand 2) (Azide free) (HRP); EPH-related receptor tyrosine kinase ligand 2; anti-EFNB1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-EFNB1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: EFNB1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 88-116 amino acids from the Central region of human EFNB1.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(EFNB1 Antibody (Center) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the EFNB1 antibody detected the EFNB1 protein (arrow).)

Related Product Information for anti-EFNB1 antibody

The protein encoded by this gene is a type I membrane protein and a ligand of Eph-related receptor tyrosine kinases. It may play a role in cell adhesion and function in the development or maintenance of the nervous system.

Product Categories/Family for anti-EFNB1 antibody

Antibodies; Protein Kinases

References

Hogue, J., et al. Am. J. Med. Genet. A 152A (10), 2574-2577 (2010): Arvanitis, D.N., et al. Mol. Cell. Biol. 30(10):2508-2517(2010). Makarov, R., et al. BMC Med. Genet. 11, 98 (2010): Vazin, T., et al. PLoS ONE 4 (8), E6606 (2009): Wallis, D., et al. Am. J. Med. Genet. A 146A (15), 2008-2012 (2008).

NCBI and Uniprot Product Information

• NCBI GI #: 4758248
• NCBI GeneID: 1947
• NCBI Accession #: NP_004420.1
• NCBI GenBank Nucleotide #: NM_004429.4
• UniProt Accession #: P98172; D3DVU0
• Molecular Weight: 38,007 Da
• NCBI Official Full Name: ephrin-B1
• NCBI Official Synonym Full Names: ephrin-B1
• NCBI Official Symbol: EFNB1
• NCBI Official Synonym Symbols: CFND; CFNS; EFL3; EPLG2; Elk-L; LERK2
• NCBI Protein Information: ephrin-B1; EFL-3; LERK-2; ELK ligand; ligand of eph-related kinase 2; eph-related receptor tyrosine kinase ligand 2
• UniProt Protein Name: Ephrin-B1
• Protein Family: Ephrin
• UniProt Gene Name: EFNB1
• UniProt Synonym Gene Names: EFL3; EPLG2; LERK2; ELK-L; LERK-2
• UniProt Entry Name: EFNB1_HUMAN

NCBI Description

The protein encoded by this gene is a type I membrane protein and a ligand of Eph-related receptor tyrosine kinases. It may play a role in cell adhesion and function in the development or maintenance of the nervous system. [provided by RefSeq, Jul 2008]

Uniprot Description

EFNB1: a type I membrane protein of the ephrin family. A ligand of Eph-related receptor tyrosine kinases EphB1 and EphA1. Ephrins and ephrin receptors mediate numerous developmental processes, particularly in the nervous system. Ephrin-B1 may play a role in cell adhesion and functions in the development or maintenance of the nervous system. Binding to its receptor induces the collapse of commissural axons/growth cones in vitro. Induced by TNF-alpha. Expressed in brain, heart, placenta, lung, liver, skeletal muscle, kidney, and pancreas.

Protein type: Membrane protein, integral; Ligand, receptor tyrosine kinase

Chromosomal Location of Human Ortholog: Xq12

Cellular Component: integral to plasma membrane; cytoplasm; plasma membrane; synapse; nucleus; lipid raft

Molecular Function: protein binding; ephrin receptor binding

Biological Process: axon guidance; cell-cell signaling; ephrin receptor signaling pathway; positive regulation of T cell proliferation; cell adhesion; embryonic pattern specification; neural crest cell migration

Disease: Craniofrontonasal Syndrome