Rabbit anti-Human eEF2k Polyclonal Antibody | anti-EEF2K antibody

eEF2k, Phosphorylated (Ser366) (eEF2k (Eucharyotic Elongation Factor-2 kinase, eEF-2 kinase)

Cat. Num. AAA612730

• Gene Names: EEF2K; eEF-2K; HSU93850; MGC45041
• Reactivity: Human
• Applications: Western Blot, Immunoprecipitation
• Purity: Affinity Purified; Purified by protein A and peptide affinity chromatography.
• Synonyms: eEF2k; Polyclonal Antibody; Phosphorylated (Ser366) (eEF2k (Eucharyotic Elongation Factor-2 kinase; eEF-2 kinase); Anti -eEF2k; anti-EEF2K antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Detects endogenous levels of eEF2k only when phosphorylated at Ser366.
• Purity/Purification: Affinity Purified; Purified by protein A and peptide affinity chromatography.
• Form/Format: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, BSA, 50% glycerol.

• Applicable Applications for anti-EEF2K antibody: Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: Suitable for use in Western Blotting, Immunoprecipitation.; Dilution: Western Blot: 1:1000; Immunoprecipitation: 1:100
• Immunogen: Synthetic phosphopeptide (KLH coupled) corresponding to residues surrounding Ser366 of human eEF2k.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot analysis of extracts from SW-13 cells (starved for 18 hours) treated with calf intestinal alkaline phosphatase (CIP) or 20% fetal bovine serum for 30 minutes, and extracts from HeLa cells (starved for 18 hours) treated with 200 nM TPA for 30 minutes, using Phospho-eEF2k (Ser366) Antibody (upper) or eEF2k Antibody MBS612730 (lower).)

Related Product Information for anti-EEF2K antibody

Eukaryotic elongation factor 2 kinase (eEF2k) phosphorylates and inactivates eEF2, resulting in the inhibition of peptide-chain elongation (1). eEF2k is normally dependent on Ca2+ ions and calmodulin (2,3). It can be activated by PKA in response to elevated cAMP levels (4-6), which are generally increased in stress-or starvation-related conditions. eEF2k can also be regulated in response to a wide range of stimuli that promote cell growth and protein synthesis. This involves the phosphorylation of eEF2k by p90RSK and p70 S6 kinase at Ser366 or by SAPK4/p38d at Ser359, leading to the inactivation of eEF2k (7,8), which facilitates the dephosphorylation of eEF2, and thus promotes translation.

Product Categories/Family for anti-EEF2K antibody

Antibodies; Abs to Cancer Markers

NCBI and Uniprot Product Information

• NCBI GI #: 9558749
• NCBI GeneID: 29904
• NCBI Accession #: NP_037434.1
• NCBI GenBank Nucleotide #: NM_013302.3
• UniProt Accession #: O00418; Q8N588
• Molecular Weight: 82,144 Da
• NCBI Official Full Name: eukaryotic elongation factor 2 kinase
• NCBI Official Synonym Full Names: eukaryotic elongation factor-2 kinase
• NCBI Official Symbol: EEF2K
• NCBI Official Synonym Symbols: eEF-2K; HSU93850; MGC45041
• NCBI Protein Information: eukaryotic elongation factor 2 kinase; eEF-2 kinase; OTTHUMP00000119998; elongation factor-2 kinase; eukaroytic elongation factor 2 kinase; calmodulin-dependent protein kinase III; calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase; calcium/calmodulin-dependent eukaryotic elongation factor-2 kinase
• UniProt Protein Name: Eukaryotic elongation factor 2 kinase
• Protein Family: Eukaryotic elongation factor 2 kinase
• UniProt Gene Name: EEF2K
• UniProt Entry Name: EF2K_HUMAN

NCBI Description

This gene encodes a highly conserved protein kinase in the calmodulin-mediated signaling pathway that links activation of cell surface receptors to cell division. This kinase is involved in the regulation of protein synthesis. It phosphorylates eukaryotic elongation factor 2 (EEF2) and thus inhibits the EEF2 function. The activity of this kinase is increased in many cancers and may be a valid target for anti-cancer treatment. [provided by RefSeq]

Uniprot Description

EEF2K: an atypical protein kinase of the Alpha family. Dependent on Ca2 ions and calmodulin. May play a key role linking cellular energy status and the inhibition of protein synthesis, a major consumer of metabolic energy. Phosphorylates and inactivates eEF2, resulting in the inhibition of peptide-chain elongation eEF2K is phosphorylated and activated by AMPK in response to elevated AMP levels, which increase during cellular stress and starvation. Regulated in response to a range of stimuli including TNF-alpha. Overexpressed in breast cancer cell lines and tumors. Activated in rat glioblastoma, and growth was inhibited by the drug Rottlerin. Activity induced in ischemic heart muscle

Protein type: EC 2.7.11.20; Kinase, protein; Protein kinase, atypical; Protein kinase, Ser/Thr (non-receptor); Translation; ATYPICAL group; Alpha family; eEF2K subfamily

Chromosomal Location of Human Ortholog: 16p12.2

Cellular Component: cytoplasm; cytosol

Molecular Function: calmodulin binding; elongation factor-2 kinase activity; translation factor activity, nucleic acid binding; calcium ion binding; ATP binding; protein kinase activity

Biological Process: translational elongation; regulation of protein amino acid autophosphorylation; protein amino acid autophosphorylation; insulin receptor signaling pathway