Testing Data
(Recombinant human eEF2K untreated (lanes 1 and 4) and phosphorylated with p38 kinase in vitro (lanes 2 & 5). After in vitro reaction, the eEF2K was dephosphorylated with lambda phosphatase (lanes 3 & 6). The blots were probed with rabbit polyclonal anti-eEF2K (N-terminus) (lanes 1-3) or anti-eEF2K (Ser-359) (lanes 4-6). (Images provided by the laboratory of Dr. Kevin Dalby in the Dept. of Pharmacy at the University of Texas at Austin.))
Rabbit eEF2K Polyclonal Antibody | anti-eEF2K antibody
eEF2K (Ser-359), phospho-specific Antibody
Reactivity
Human, Rat, Mouse
Applications
Western Blot, ELISA
Synonyms
eEF2K; Polyclonal Antibody; eEF2K (Ser-359); phospho-specific Antibody; phospho-specific; eEF2K 359; anti-eEF2K antibody
Host
Rabbit
Reactivity
Human, Rat, Mouse
Clonality
Polyclonal
Specificity
The antibody was affinity purified using phospho-eEF2K (Ser-359) peptide (without carrier). This antibody detects a 105kDa* protein corresponding to the molecular mass of eEF2K on SDS-PAGE immunoblots of human recombinant eEF2K phosphorylated with p38 MAP kinase. This reactivity is removed after lambda phosphatase treatment.
Form/Format
Rabbit polyclonal, affinity-purified antibody is supplied in 100ul phosphate-buffered saline, 50% glycerol, 1mg/ml BSA, and 0.05% sodium azide.
Applicable Applications for anti-eEF2K antibody
Western Blot (WB), ELISA (EIA)
Application Notes
ELISA: 1:2000
WB: 1:1000
WB: 1:1000
Immunogen
Phospho-eEF2K (Ser-359) synthetic peptide (coupled to carrier protein) corresponds to amino acids surrounding Ser-359 in human eEF2K. This sequence is well conserved in rat and mouse eEF2K, and has low homology to other proteins.
Preparation and Storage
Store at -20 degree C. Stable for 1 year.
Testing Data
(Recombinant human eEF2K untreated (lanes 1 and 4) and phosphorylated with p38 kinase in vitro (lanes 2 & 5). After in vitro reaction, the eEF2K was dephosphorylated with lambda phosphatase (lanes 3 & 6). The blots were probed with rabbit polyclonal anti-eEF2K (N-terminus) (lanes 1-3) or anti-eEF2K (Ser-359) (lanes 4-6). (Images provided by the laboratory of Dr. Kevin Dalby in the Dept. of Pharmacy at the University of Texas at Austin.))
Testing Data
(Recombinant human eEF2K untreated (lanes 1 and 4) and phosphorylated with p38 kinase in vitro (lanes 2 & 5). After in vitro reaction, the eEF2K was dephosphorylated with lambda phosphatase (lanes 3 & 6). The blots were probed with rabbit polyclonal anti-eEF2K (N-terminus) (lanes 1-3) or anti-eEF2K (Ser-359) (lanes 4-6). (Images provided by the laboratory of Dr. Kevin Dalby in the Dept. of Pharmacy at the University of Texas at Austin.))
Related Product Information for anti-eEF2K antibody
Eukaryotic elongation factor 2 (eEF2) catalyzes the translocation of peptidyl-tRNA from the A site to the P site on the ribosome. eEF2 kinase (eEF2K) phosphorylates and inactivates eEF2, resulting in the inhibition of peptide-chain elongation. eEF2K is normally dependent on Ca2+ ions and calmodulin, and can be activated by PKA in response to elevated cAMP levels during cell stress- or starvation-related conditions. Regulation of eEF2K occurs through phosphorylation at multiple sites. Ser-78 phosphorylation is required for calmodulin binding and eEF2K activity, while phosphorylation of Ser-500 is required for Ca2+/calmodulin-independent kinase activity. Thr-348 is an autophosphorylation site that is required for kinase activity. Inhibitory phosphorylation may also regulate eEF2K, since phosphorylation at Ser-359 by SAPK4/p38delta causes inactivation of eEF2K. Thus, multisite phospho-regulation of eEF2K may be important for proper control of eEF2K activity and protein translation.
References
Tavares, C.D. et al. (2012) Biochemistry. 51(11):2232.
Smith, E.M. & Proud, C.G. (2008) EMBO J. 27(7):1005.
Ryazanov, A.G. et al. (1997) Proc Natl Acad Sci. USA. 94:4884.
Smith, E.M. & Proud, C.G. (2008) EMBO J. 27(7):1005.
Ryazanov, A.G. et al. (1997) Proc Natl Acad Sci. USA. 94:4884.
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Product Notes
The eEF2K (Catalog #AAA474346) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The eEF2K (Ser-359), phospho-specific Antibody reacts with Human, Rat, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's eEF2K can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). ELISA: 1:2000 WB: 1:1000. Researchers should empirically determine the suitability of the eEF2K for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "eEF2K, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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