Rabbit EEF2K Polyclonal Antibody | anti-EEF2K antibody

Anti-EEF2K Antibody (aa336-385) IHC-plus

Cat. Num. AAA249753

• Gene Names: EEF2K; eEF-2K; HSU93850
• Reactivity: Mouse, Rat, Human
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Immunoaffinity purified
• Synonyms: EEF2K; Polyclonal Antibody; Anti-EEF2K Antibody (aa336-385) IHC-plus; Rabbit Polyclonal (IgG) to Human EEF2K; Human EEF2K; EEF-2K; Elongation factor-2 kinase; EEF-2 kinase; HSU93850; anti-EEF2K antibody

• Host: Rabbit
• Reactivity: Mouse, Rat, Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: eEF2K Antibody detects endogenous levels of total eEF2K protein.
• Purity/Purification: Immunoaffinity purified
• Form/Format: PBS (without Mg2+, Ca2+), pH 7.4, 150 mM sodium chloride, 0.02% sodium azide, 50% glycerol
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 725

• Applicable Applications for anti-EEF2K antibody: Immunohistochemistry (IHC) Paraffin, Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA (1:40000); IHC-P (10 ug/ml); WB (1:500 - 1:1000)
• Immunogen: EEF2K antibody was raised against synthetic peptide from human eEF2K (aa336-385).
• Immunogen Description: Synthetic peptide from human eEF2K (aa336-385).
• Antigen Modification: aa336-385
• Target Species: Human
• Preparation and Storage: Store at -20 degree C for up to one year.

Immunohistochemistry (IHC)

(Anti-EEF2K antibody IHC staining of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Western Blot (WB)

(Western blot of extracts from Jurkat cells, using eEF2K Antibody. The lane on the right is treated with the synthesized peptide.)

Related Product Information for anti-EEF2K antibody

EEF2K Antibody, EEF-2K Antibody, Elongation factor-2 kinase Antibody, EEF-2 kinase Antibody, HSU93850 Antibody Description: EEF2K, also known as Ca2+/CaM-dependent protein kinase III, is a Alpha-kinase. EEF2K, a member of the calmodulin-mediated signaling pathway, catalyzes the movement of the ribosome along mRNA during translation in eukaryotic cells. At least three mRNA variants have been reported -- a major 3.1-kb transcript and minor 6.1- and 2.5-kb transcripts. EEF2K activity has been shown to be increased in several types of cancer, including breast cancer.

NCBI and Uniprot Product Information

• NCBI GI #: 9558749
• NCBI GeneID: 29904
• NCBI Accession #: NP_037434.1
• NCBI GenBank Nucleotide #: NM_013302.3
• UniProt Accession #: O00418; Q8N588
• Molecular Weight: 82,144 Da
• NCBI Official Full Name: eukaryotic elongation factor 2 kinase
• NCBI Official Synonym Full Names: eukaryotic elongation factor 2 kinase
• NCBI Official Symbol: EEF2K
• NCBI Official Synonym Symbols: eEF-2K; HSU93850
• NCBI Protein Information: eukaryotic elongation factor 2 kinase
• UniProt Protein Name: Eukaryotic elongation factor 2 kinase
• Protein Family: Eukaryotic elongation factor 2 kinase
• UniProt Gene Name: EEF2K
• UniProt Synonym Gene Names: eEF-2 kinase; eEF-2K
• UniProt Entry Name: EF2K_HUMAN

NCBI Description

This gene encodes a highly conserved protein kinase in the calmodulin-mediated signaling pathway that links activation of cell surface receptors to cell division. This kinase is involved in the regulation of protein synthesis. It phosphorylates eukaryotic elongation factor 2 (EEF2) and thus inhibits the EEF2 function. The activity of this kinase is increased in many cancers and may be a valid target for anti-cancer treatment. [provided by RefSeq, Jul 2008]

Uniprot Description

EEF2K: an atypical protein kinase of the Alpha family. Dependent on Ca2 ions and calmodulin. May play a key role linking cellular energy status and the inhibition of protein synthesis, a major consumer of metabolic energy. Phosphorylates and inactivates eEF2, resulting in the inhibition of peptide-chain elongation eEF2K is phosphorylated and activated by AMPK in response to elevated AMP levels, which increase during cellular stress and starvation. Regulated in response to a range of stimuli including TNF-alpha. Overexpressed in breast cancer cell lines and tumors. Activated in rat glioblastoma, and growth was inhibited by the drug Rottlerin. Activity induced in ischemic heart muscle

Protein type: EC 2.7.11.20; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; Protein kinase, atypical; Translation; ATYPICAL group; Alpha family; eEF2K subfamily

Chromosomal Location of Human Ortholog: 16p12.2

Cellular Component: cytoplasm; cytosol; postsynaptic density

Molecular Function: ATP binding; calcium ion binding; calmodulin binding; elongation factor-2 kinase activity; protein kinase activity; translation factor activity, nucleic acid binding

Biological Process: insulin receptor signaling pathway; negative regulation of apoptosis; positive regulation of endocytosis; positive regulation of synaptogenesis; protein amino acid autophosphorylation; regulation of protein amino acid autophosphorylation; translational elongation