Rabbit EEF2K Polyclonal Antibody | anti-EEF2K antibody

EEF2K, NT (Elongation factor 2 kinase, eEF-2 kinase, eEF-2K)

Cat. Num. AAA6011773

• Gene Names: EEF2K; eEF-2K; HSU93850
• Reactivity: Human, Mouse, Rat, Th
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: EEF2K; Polyclonal Antibody; NT (Elongation factor 2 kinase; eEF-2 kinase; eEF-2K); Anti -EEF2K; anti-EEF2K antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat, Th
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human EEF2. Species Crossreactivity: Mouse,rat.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, (without Mg2+, Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.

• Applicable Applications for anti-EEF2K antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: Suitable for use in Immunofluorescence, ELISA, Western Blot, Immunohistochemistry.; Dilution: Immunofluorescence: 1:100-1:500; ELISA: 1:5000; Western Blot: 1:500-1:1000; Immunohistochemistry (Paraffin): 10ug/ml
• Immunogen: Synthetic peptide corresponding to human EEF2.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(EEF2 IHC staining of human prostate. Immunohistochemistry of formalin- fixed, paraffin-embedded tissue after heat-induced retrieval.)

Western Blot (WB)

(Western blot analysis of extracts from NIH-3T3 cells, treated with serum 10% 30', using eEF2. The lane on the right is treated with the synthesized peptide.)

Immunofluorescence (IF)

(Immunofluorescence analysis of HuvEc cells, using eEF2 .The picture on the right is treated with the synthesized peptide.)

Related Product Information for anti-EEF2K antibody

This gene encodes a member of the GTP-binding translation elongation factor family. This protein is an essential factor for protein synthesis. It promotes the GTP-dependent translocation of the nascent protein chain from the A-site to the P-site of the ribosome. This protein is completely inactivated by EF-2 kinase phosporylation.

Product Categories/Family for anti-EEF2K antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 9558749
• NCBI GeneID: 29904
• NCBI Accession #: NP_037434.1
• NCBI GenBank Nucleotide #: NM_013302.3
• UniProt Accession #: O00418; Q8N588
• Molecular Weight: 82,144 Da
• NCBI Official Full Name: eukaryotic elongation factor 2 kinase
• NCBI Official Synonym Full Names: eukaryotic elongation factor-2 kinase
• NCBI Official Symbol: EEF2K
• NCBI Official Synonym Symbols: eEF-2K; HSU93850
• NCBI Protein Information: eukaryotic elongation factor 2 kinase; eEF-2 kinase; elongation factor-2 kinase; eukaroytic elongation factor 2 kinase; calmodulin-dependent protein kinase III; calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase; calcium/calmodulin-dependent eukaryotic elongation factor-2 kinase
• UniProt Protein Name: Eukaryotic elongation factor 2 kinase
• Protein Family: Eukaryotic elongation factor 2 kinase
• UniProt Gene Name: EEF2K
• UniProt Synonym Gene Names: eEF-2 kinase; eEF-2K
• UniProt Entry Name: EF2K_HUMAN

NCBI Description

This gene encodes a highly conserved protein kinase in the calmodulin-mediated signaling pathway that links activation of cell surface receptors to cell division. This kinase is involved in the regulation of protein synthesis. It phosphorylates eukaryotic elongation factor 2 (EEF2) and thus inhibits the EEF2 function. The activity of this kinase is increased in many cancers and may be a valid target for anti-cancer treatment. [provided by RefSeq, Jul 2008]

Uniprot Description

EEF2K: an atypical protein kinase of the Alpha family. Dependent on Ca2 ions and calmodulin. May play a key role linking cellular energy status and the inhibition of protein synthesis, a major consumer of metabolic energy. Phosphorylates and inactivates eEF2, resulting in the inhibition of peptide-chain elongation eEF2K is phosphorylated and activated by AMPK in response to elevated AMP levels, which increase during cellular stress and starvation. Regulated in response to a range of stimuli including TNF-alpha. Overexpressed in breast cancer cell lines and tumors. Activated in rat glioblastoma, and growth was inhibited by the drug Rottlerin. Activity induced in ischemic heart muscle

Protein type: EC 2.7.11.20; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; Translation; Protein kinase, atypical; ATYPICAL group; Alpha family; eEF2K subfamily

Chromosomal Location of Human Ortholog: 16p12.2

Cellular Component: cytoplasm; cytosol

Molecular Function: calmodulin binding; elongation factor-2 kinase activity; translation factor activity, nucleic acid binding; calcium ion binding; ATP binding; protein kinase activity

Biological Process: translational elongation; regulation of protein amino acid autophosphorylation; protein amino acid autophosphorylation; insulin receptor signaling pathway