Rabbit anti-Human EEF1D Polyclonal Antibody | anti-EEF1D antibody

EEF1D, NT (Elongation Factor 1-delta, EF-1-delta, Antigen NY-CO-4, EF1D) (MaxLight 405)

Cat. Num. AAA6473579

• Gene Names: EEF1D; EF1D; EF-1D; FP1047
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: EEF1D; Polyclonal Antibody; NT (Elongation Factor 1-delta; EF-1-delta; Antigen NY-CO-4; EF1D) (MaxLight 405); anti-EEF1D antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human EEF1D.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight405.

• Applicable Applications for anti-EEF1D antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:50-1:100; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the N-terminal region of human EEF1D (KLH).
• Conjugate: MaxLight405
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight405 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of EEF1D antibody (N-term) in Ramos cell line lysates (35ug/lane). EEF1D (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human lymph reacted with EEF1D Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(EEF1D Antibody (N-term) flow cytometry analysis of Ramos cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-EEF1D antibody

Antibodies; Cancer Markers

References

1. Yang, S., BMC Cancer 7, 211 (2007). 2. Mulner-Lorillon, O., J. Biol. Chem. 269(31), 20201-20207 (1994).

NCBI and Uniprot Product Information

• NCBI GI #: 25453472
• NCBI GeneID: 1936
• NCBI Accession #: NP_001951
• NCBI GenBank Nucleotide #: NM_001960.5
• UniProt Accession #: P29692; Q4VBZ6; Q969J1; Q96I38; B4DDU4; D3DWK3; E9PBQ9
• Molecular Weight: 33.2 kDa (301aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)
• NCBI Official Full Name: elongation factor 1-delta isoform 2
• NCBI Official Synonym Full Names: eukaryotic translation elongation factor 1 delta
• NCBI Official Symbol: EEF1D
• NCBI Official Synonym Symbols: EF1D; EF-1D; FP1047
• NCBI Protein Information: elongation factor 1-delta
• UniProt Protein Name: Elongation factor 1-delta
• Protein Family: Elongation factor
• UniProt Gene Name: EEF1D
• UniProt Synonym Gene Names: EF1D; EF-1-delta
• UniProt Entry Name: EF1D_HUMAN

NCBI Description

This gene encodes a subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This subunit, delta, functions as guanine nucleotide exchange factor. It is reported that following HIV-1 infection, this subunit interacts with HIV-1 Tat. This interaction results in repression of translation of host cell proteins and enhanced translation of viral proteins. Several alternatively spliced transcript variants encoding multiple isoforms have been found for this gene. Related pseudogenes have been defined on chromosomes 1, 6, 7, 9, 11, 13, 17, 19.[provided by RefSeq, Aug 2010]

Uniprot Description

EEF1D: EF-1-beta and EF-1-delta stimulate the exchange of GDP bound to EF-1-alpha to GTP. Belongs to the EF-1-beta/EF-1-delta family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Translation elongation; Translation

Chromosomal Location of Human Ortholog: 8q24.3

Cellular Component: eukaryotic translation elongation factor 1 complex; endoplasmic reticulum; cytoplasm; nucleolus; nucleus; cytosol

Molecular Function: signal transducer activity; protein binding; transcription activator binding; DNA binding; translation factor activity, nucleic acid binding; heat shock protein binding; translation elongation factor activity

Biological Process: cellular protein metabolic process; translational elongation; positive regulation of I-kappaB kinase/NF-kappaB cascade; regulation of transcription, DNA-dependent; translation; transcription, DNA-dependent; mRNA transcription; gene expression; signal transduction