Western Blot (WB)
(Western blot analysis of extracts from rat muscle, using EEF1A2 Antibody.)
Rabbit EEF1A2 Polyclonal Antibody | anti-EEF1A2 antibody
EEF1A2 Antibody
Gene Names
EEF1A2; HS1; STN; EF1A; STNL; MRD38; EEF1AL; EIEE33; EF-1-alpha-2
Reactivity
Human, Mouse, Rat
Applications
Western Blot, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
EEF1A2; Polyclonal Antibody; EEF1A2 Antibody; CCS3; eEF1A-1; eEF1A-2; EEF1A1; Eef1a2; EEF1AL; EF 1 alpha 2; EF-1-alpha-1; EF-1-alpha-2; EF-Tu; EF1A; EF1A1_HUMAN; EF1A2_HUMAN; Elongation factor 1 A 2; Elongation factor 1 alpha; Elongation factor 1 alpha 2; Elongation factor 1-alpha 1; Elongation factor 1-alpha 2; Elongation factor Tu; Eukaryotic elongation factor 1 A-1; Eukaryotic elongation factor 1 A-2; Eukaryotic translation elongation factor 1 alpha 2; GRAF 1EF; HS1; Leukocyte receptor cluster member 7; PTI1; Statin-S1; anti-EEF1A2 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
EEF1A2 antibody detects endogenous levels of total EEF1A2
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
463
Applicable Applications for anti-EEF1A2 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:1000-3000
Immunogen
A synthesized peptide derived from human EEF1A2
Subcellular Location
Nucleus.
Tissue Specificity
Brain, heart, and skeletal muscle.
Predicted Cross Reactivity
Pig, Bovine, Horse, Sheep, Rabbit, Chicken, Xenopus
Similarity
Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Chicken (100%), Xenopus (91%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of extracts from rat muscle, using EEF1A2 Antibody.)
Western Blot (WB)
(Western blot analysis of extracts from rat muscle, using EEF1A2 Antibody.)
Western Blot (WB)
(Western blot analysis of EEF1A2 using COLO205 whole cell lysates)
Western Blot (WB)
(Western blot analysis of EEF1A2 using COLO205 whole cell lysates)
Related Product Information for anti-EEF1A2 antibody
Function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.
Subunit Structure: Monomer.
Post-translational Modifications: Trimethylated at Lys-165 by EEF1AKMT3 (PubMed:28108655). Mono-, di-, and trimethylated at Lys-36 by EEF1AKMT4; trimethylated form is predominant. Methylation by EEF1AKMT4 contributes to the fine-tuning of translation rates for a subset of tRNAs (PubMed:28520920).
Similarity: Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A subfamily.
Subunit Structure: Monomer.
Post-translational Modifications: Trimethylated at Lys-165 by EEF1AKMT3 (PubMed:28108655). Mono-, di-, and trimethylated at Lys-36 by EEF1AKMT4; trimethylated form is predominant. Methylation by EEF1AKMT4 contributes to the fine-tuning of translation rates for a subset of tRNAs (PubMed:28520920).
Similarity: Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A subfamily.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 50 kDa
Predicted: 51 kDa
Predicted: 51 kDa
NCBI Official Full Name
elongation factor 1-alpha 2
NCBI Official Synonym Full Names
eukaryotic translation elongation factor 1 alpha 2
NCBI Official Symbol
EEF1A2
NCBI Official Synonym Symbols
HS1; STN; EF1A; STNL; MRD38; EEF1AL; EIEE33; EF-1-alpha-2
NCBI Protein Information
elongation factor 1-alpha 2
UniProt Protein Name
Elongation factor 1-alpha 2
UniProt Gene Name
EEF1A2
UniProt Synonym Gene Names
EEF1AL; STN; EF-1-alpha-2; eEF1A-2
NCBI Description
This gene encodes an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This isoform (alpha 2) is expressed in brain, heart and skeletal muscle, and the other isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas. This gene may be critical in the development of ovarian cancer. [provided by RefSeq, Mar 2014]
Uniprot Description
This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.
Research Articles on EEF1A2
Similar Products
Product Notes
The EEF1A2 eef1a2 (Catalog #AAA9609810) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The EEF1A2 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's EEF1A2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:1000-3000. Researchers should empirically determine the suitability of the EEF1A2 eef1a2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EEF1A2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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