Western Blot (WB)
((0.5ug/ml) staining of Jurkat lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat EEF1A2 Polyclonal Antibody | anti-EEF1A2 antibody
Goat anti-EEF1A2 Antibody
Western Blot: Approx 50kDa band observed in lysates of cell lines Daudi and Jurkat (calculated MW of 50.5kDa according to NP_001949.1). The same band is observed in lysates of Mouse and Rat Heart and Skeletal Muscle. Recommended concentration: 0.5-2ug/ml.
Western Blot (WB)
((0.5ug/ml) staining of Jurkat lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0.5ug/ml) staining of Jurkat lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0.5ug/ml) staining of Mouse (A+C) and Rat (B+D) Skeletal Muscle (A+B) and Heart (C+D) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0.5ug/ml) staining of Mouse (A+C) and Rat (B+D) Skeletal Muscle (A+B) and Heart (C+D) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This isoform (alpha 2) is expressed in brain, heart and skeletal muscle, and the other isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas. This gene may be critical in the development of ovarian cancer. [provided by RefSeq, Mar 2014]
Uniprot Description
EEF1A2: an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This isoform (alpha 2) is expressed in brain, heart and skeletal muscle, and the other isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas. This gene may be critical in the development of ovarian cancer. Promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.
Protein type: Translation elongation; Translation
Chromosomal Location of Human Ortholog: 20q13.3
Cellular Component: cell soma; cytoplasm; eukaryotic translation elongation factor 1 complex; myelin sheath; nucleus
Molecular Function: GTP binding; GTPase activity; protein binding; protein kinase binding; translation elongation factor activity; translation factor activity, nucleic acid binding
Biological Process: positive regulation of apoptosis; response to electrical stimulus; response to inorganic substance; translational elongation
Disease: Epileptic Encephalopathy, Early Infantile, 33; Mental Retardation, Autosomal Dominant 38
Research Articles on EEF1A2
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Product Notes
The EEF1A2 eef1a2 (Catalog #AAA423152) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-EEF1A2 Antibody reacts with Tested: Human. Mouse, Rat; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's EEF1A2 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 8000. Western Blot: Approx 50kDa band observed in lysates of cell lines Daudi and Jurkat (calculated MW of 50.5kDa according to NP_001949.1). The same band is observed in lysates of Mouse and Rat Heart and Skeletal Muscle. Recommended concentration: 0.5-2ug/ml. Researchers should empirically determine the suitability of the EEF1A2 eef1a2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: NPATVPFVPI S. It is sometimes possible for the material contained within the vial of "EEF1A2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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