Rabbit anti-Human, Mouse EDN3 Polyclonal Antibody | anti-EDN3 antibody

EDN3 antibody

Cat. Num. AAA9405186

• Gene Names: EDN3; ET3; ET-3; WS4B; HSCR4; PPET3
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Antibodies were purified by affinity purification using immunogen.
• Synonyms: EDN3; Polyclonal Antibody; EDN3 antibody; ET3; MGC15067; MGC61498; anti-EDN3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of total EDN3 antibody.
• Purity/Purification: Antibodies were purified by affinity purification using immunogen.
• Form/Format: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 198

• Applicable Applications for anti-EDN3 antibody: Western Blot (WB)
• Application Notes: Western blotting: 1:500 - 1:2000
• Preparation and Storage: Store at -20°C

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using EDN3 antibody.)

Related Product Information for anti-EDN3 antibody

Background/Introduction: The product is a loading buffer for protein samples with SDS-PAGE electrophoresis. The SDS contained in the product can be combined with the protein to form a SDS-protein complex, which bring a large amount of negative charge to the protein; SDS can break intramolecular and intermolecular hydrogen bonds, and destroy the secondary and the tertiary structure of protein. The DTT contained in the preparation can break the disufide bond between the cysteine residues, destroy the structure between the proteins, and eliminate the difference between the protein structures. Ultimately, the rate of protein migration in the SDS-PAGE is only related to its molecular weight. Bromophenol blue is used as an indicator for electrophoresis to determine the progress of electrophoresis.

Product Categories/Family for anti-EDN3 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 698848571
• NCBI GeneID: 1908
• NCBI Accession #: NP_001289384.1
• NCBI GenBank Nucleotide #: NM_001302455.1
• UniProt Accession #: P14138; Q03229; Q7Z6D2; Q9UGT7; E1P5I5
• NCBI Official Full Name: endothelin-3 isoform 4 preproprotein
• NCBI Official Synonym Full Names: endothelin 3
• NCBI Official Symbol: EDN3
• NCBI Official Synonym Symbols: ET3; ET-3; WS4B; HSCR4; PPET3
• NCBI Protein Information: endothelin-3
• UniProt Protein Name: Endothelin-3
• Protein Family: Endothelin
• UniProt Gene Name: EDN3
• UniProt Synonym Gene Names: ET-3; PPET3
• UniProt Entry Name: EDN3_HUMAN

NCBI Description

The protein encoded by this gene is a member of the endothelin family. Endothelins are endothelium-derived vasoactive peptides involved in a variety of biological functions. The active form of this protein is a 21 amino acid peptide processed from the precursor protein. The active peptide is a ligand for endothelin receptor type B (EDNRB). The interaction of this endothelin with EDNRB is essential for development of neural crest-derived cell lineages, such as melanocytes and enteric neurons. Mutations in this gene and EDNRB have been associated with Hirschsprung disease (HSCR) and Waardenburg syndrome (WS), which are congenital disorders involving neural crest-derived cells. Altered expression of this gene is implicated in tumorigenesis. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Oct 2014]

Uniprot Description

EDN3: Endothelins are endothelium-derived vasoconstrictor peptides. Defects in EDN3 are the cause of Hirschsprung disease type 4 (HSCR4); also known as aganglionic megacolon (MGC). A genetic disorder of neural crest development characterized by the absence of intramural ganglion cells in the hindgut; often resulting in intestinal obstruction. Defects in EDN3 are a cause of congenital central hypoventilation syndrome (CCHS); also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia. Defects in EDN3 are a cause of Waardenburg syndrome type 4 (WS4B); also known as Waardenburg-Shah syndrome. WS4B is characterized by the association of Waardenburg features (depigmentation and deafness) and the absence of enteric ganglia in the distal part of the intestine (Hirschsprung disease). Belongs to the endothelin/sarafotoxin family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 20q13.2-q13.3

Cellular Component: extracellular space; extracellular region

Molecular Function: hormone activity; receptor binding

Biological Process: vasoconstriction; neutrophil chemotaxis; inositol phosphate-mediated signaling; regulation of systemic arterial blood pressure by endothelin; positive regulation of mitosis; blood circulation; multicellular organismal development; positive regulation of hormone secretion; positive regulation of heart rate; positive regulation of leukocyte chemotaxis; vein smooth muscle contraction; peptide hormone secretion; signal transduction; neuron differentiation; cellular calcium ion homeostasis; positive regulation of MAP kinase activity; cell surface receptor linked signal transduction; cell-cell signaling; regulation of gene expression; regulation of vasoconstriction; melanocyte differentiation; positive regulation of cell proliferation; regulation of pigmentation during development; positive regulation of cell differentiation; neural crest cell migration; artery smooth muscle contraction

Disease: Hirschsprung Disease, Susceptibility To, 4; Waardenburg Syndrome, Type 4b; Central Hypoventilation Syndrome, Congenital