Rabbit anti-Human, Mouse EDG3 Polyclonal Antibody | anti-EDG3 antibody

EDG3, NT (Sphingosine 1-phosphate Receptor 3, S1P Receptor 3, S1P3, Endothelial Differentiation G-protein Coupled Receptor 3, Sphingosine 1-phosphate Receptor Edg-3, S1P Receptor Edg-3, S1PR3) (Azide free) (HRP)

Cat. Num. AAA6500418

• Gene Names: S1PR3; EDG3; LPB3; S1P3; EDG-3
• Reactivity: Human, Mouse
• Applications: Western Blot, ELISA
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: EDG3; Polyclonal Antibody; NT (Sphingosine 1-phosphate Receptor 3; S1P Receptor 3; S1P3; Endothelial Differentiation G-protein Coupled Receptor 3; Sphingosine 1-phosphate Receptor Edg-3; S1P Receptor Edg-3; S1PR3) (Azide free) (HRP); anti-EDG3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human EDG3. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-EDG3 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-1:500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the N-terminal region of human EDG3 (KLH).
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(The anti-EDG3 N-term Antibody is used in Western blot to detect EDG3 in mouse kidney lysate.)

Product Categories/Family for anti-EDG3 antibody

Antibodies; G Protein Receptors

References

1. Licht, T., et al., Blood 102(6):2099-2107 (2003). 2. Himmel, H.M., et al., Mol. Pharmacol. 58(2):449-454 (2000). 3. An, S., et al., J. Biol. Chem. 275(1):288-296 (2000). 4. Ancellin, N., et al., J. Biol. Chem. 274(27):18997-19002 (1999). 5. Lee, M.J., et al., Cell 99(3):301-312 (1999).

NCBI and Uniprot Product Information

• NCBI GI #: 38788193
• NCBI GeneID: 1903
• NCBI Accession #: NP_005217.2
• NCBI GenBank Nucleotide #: NM_005226.3
• UniProt Accession #: Q99500; Q5SQD8; Q7Z5I2
• Molecular Weight: 42,250 Da
• NCBI Official Full Name: sphingosine 1-phosphate receptor 3
• NCBI Official Synonym Full Names: sphingosine-1-phosphate receptor 3
• NCBI Official Symbol: S1PR3
• NCBI Official Synonym Symbols: EDG3; LPB3; S1P3; EDG-3
• NCBI Protein Information: sphingosine 1-phosphate receptor 3; S1P receptor 3; S1P receptor EDG3; S1P receptor Edg-3; sphingosine 1-phosphate receptor Edg-3; endothelial differentiation G-protein coupled receptor 3; G protein-coupled receptor, endothelial differentiation gene-3; en
• UniProt Protein Name: Sphingosine 1-phosphate receptor 3
• UniProt Gene Name: S1PR3
• UniProt Synonym Gene Names: EDG3; S1P receptor 3; S1P3
• UniProt Entry Name: S1PR3_HUMAN

NCBI Description

This gene encodes a member of the EDG family of receptors, which are G protein-coupled receptors. This protein has been identified as a functional receptor for sphingosine 1-phosphate and likely contributes to the regulation of angiogenesis and vascular endothelial cell function. [provided by RefSeq, Jul 2008]

Uniprot Description

S1PR3: Receptor for the lysosphingolipid sphingosine 1- phosphate (S1P). S1P is a bioactive lysophospholipid that elicits diverse physiological effect on most types of cells and tissues. When expressed in rat HTC4 hepatoma cells, is capable of mediating S1P-induced cell proliferation and suppression of apoptosis. Belongs to the G-protein coupled receptor 1 family.

Protein type: Membrane protein, multi-pass; Membrane protein, integral; Receptor, GPCR; GPCR, family 1

Chromosomal Location of Human Ortholog: 9q22.1-q22.2

Cellular Component: integral to plasma membrane; plasma membrane

Molecular Function: G-protein coupled receptor activity; integrin binding; lipid binding

Biological Process: G-protein coupled receptor protein signaling pathway; anatomical structure morphogenesis; elevation of cytosolic calcium ion concentration; regulation of interleukin-1 beta production; positive regulation of cell proliferation; cytokine production; G-protein signaling, adenylate cyclase inhibiting pathway; inflammatory response