Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using NT5E antibody)
Rabbit Ecto-5' nucleotidase Polyclonal Antibody | anti-E5NT antibody
Ecto-5' nucleotidase (NT5E, CD73, E5NT, NT, NT5, NTE, eN, Ent) (HRP)
WB: 1:500-1:2000
IHC: 1:50-1:200
Applications are based on unconjugated antibody.
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using NT5E antibody)
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using NT5E antibody)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded mouse lung using NT5E antibody at dilution of 1:100 (400x lens).)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded mouse lung using NT5E antibody at dilution of 1:100 (400x lens).)
Immunofluorescence (IF)
(Immunofluorescence analysis of MCF7 cell using NT5E antibody. Blue: DAPI for nuclear staining.)
Immunofluorescence (IF)
(Immunofluorescence analysis of MCF7 cell using NT5E antibody. Blue: DAPI for nuclear staining.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a plasma membrane protein that catalyzes the conversion of extracellular nucleotides to membrane-permeable nucleosides. The encoded protein is used as a determinant of lymphocyte differentiation. Defects in this gene can lead to the calcification of joints and arteries. Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2011]
Uniprot Description
NT5E: Hydrolyzes extracellular nucleotides into membrane permeable nucleosides. Homodimer; disulfide-linked. Belongs to the 5'-nucleotidase family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 3.1.3.5; Membrane protein, integral; Nucleotide Metabolism - pyrimidine; Nucleotide Metabolism - purine; Phosphatase (non-protein); Membrane protein, GPI anchor; Cofactor and Vitamin Metabolism - nicotinate and nicotinamide
Chromosomal Location of Human Ortholog: 6q14-q21
Cellular Component: cell surface; membrane; cytoplasm; plasma membrane
Molecular Function: 5'-nucleotidase activity; metal ion binding; nucleotide binding
Biological Process: pyrimidine nucleoside catabolic process; dephosphorylation; pyrimidine base metabolic process; nucleobase, nucleoside and nucleotide metabolic process; adenosine biosynthetic process; negative regulation of inflammatory response; purine nucleotide catabolic process; DNA metabolic process; purine base metabolic process; AMP catabolic process
Disease: Calcification Of Joints And Arteries
Research Articles on E5NT
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Product Notes
The E5NT nt5e (Catalog #AAA6414950) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Ecto-5' nucleotidase (NT5E, CD73, E5NT, NT, NT5, NTE, eN, Ent) (HRP) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Ecto-5' nucleotidase can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC). IF: 1:50-1:200 WB: 1:500-1:2000 IHC: 1:50-1:200 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the E5NT nt5e for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Ecto-5' nucleotidase, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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