Rabbit anti-Human ECH1 Polyclonal Antibody | anti-ECH1 antibody

Anti-ECH1 Antibody, Rabbit Polyclonal

Cat. Num. AAA8107412

• Gene Names: ECH1; HPXEL
• Reactivity: Human
• Applications: Western Blot, ELISA, Immunohistochemistry, Immunoprecipitation
• Purity: Protein A & Antigen Affinity
• Synonyms: ECH1; Polyclonal Antibody; Anti-ECH1 Antibody; Rabbit Polyclonal; ECH1 Antibody; Rabbit PAb; Antigen Affinity Purified; enoyl CoA hydratase 1; peroxisomal; Anti-HPXEL Antibody; anti-ECH1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human ECH1
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-ECH1 antibody: Western Blot (WB), ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:2000; ELISA: 1:5000-1:10,000; IHC-P: 1:2500-1:10,000; IP: 0.5-2uL/mg of lysate
• Immunogen: Recombinant Human ECH1 protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Human ECH1 (rh ECH1). ECH1 specific IgG was purified by Human ECH1 affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunochemical staining of human ECH1 in human prostate with rabbit polyclonal antibody (1:10000, formalin-fixed paraffin embedded sections).)

Immunohistochemistry (IHC)

(Immunochemical staining of human ECH1 in human liver(cirrhosis) with rabbit polyclonal antibody (1:10000, formalin-fixed paraffin embedded sections).Positive staining was localized to cytoplasm of hepatocyte)

Immunoprecipitation (IP)

(ECH1 was immunoprecipitated using:Lane A:0.5 mg HepG2 Whole Cell Lysate2 uL anti-ECH1 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-ECH1 rabbit polyclonal antibody,at 1:200 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 34 kDaObserved band size: 34 kDa)

Western Blot (WB)

(Anti-ECH1 rabbit polyclonal antibody at 1:500 dilutionLane A: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:36 kDaObserved band size:34 kDa)

Related Product Information for anti-ECH1 antibody

ECH1 is a member of the hydratase/isomerase superfamily. ECH1 shows high sequence similarity to enoyl-CoA hydratases of several species, particularly within a conserved domain characteristic of these proteins. ECH1 contains a C-terminal peroxisomal targeting sequence and localizes to peroxisomes. The rat ortholog, which localizes to the matrix of both the peroxisome and mitochondria, can isomerize 3-trans, 5-cis-dienoyl-CoA to 2-trans, 4-trans-dienoyl-CoA, indicating that it is a delta3, 5-delta2, 4-dienoyl-CoA isomerase. ECH1 functions in the auxiliary step of the fatty acid beta-oxidation pathway. Expression of the rat gene is induced by peroxisome proliferators.

References

Kovalyov LI, et al. (2006) Polymorphism of delta3, 5-delta2, 4-dienoyl-coenzyme A isomerase (the ECH1 gene product protein) in human striated muscle tissue. Biochemistry Mosc. 71(4): 448-53. Olsen JV, et al. (2006) Global, in vivo, and site-specific phosphorylation dynamics in signaling networks. Cell. 127(3):635-48. FitzPatrick DR, et al. (1995) Isolation and characterization of rat and human cDNAs encoding a novel putative peroxisomal enoyl-CoA hydratase. Genomics. 27(3):457-66.

NCBI and Uniprot Product Information

• NCBI GI #: 70995211
• NCBI GeneID: 1891
• NCBI Accession #: NP_001389.2
• NCBI GenBank Nucleotide #: NM_001398.2
• UniProt Accession #: Q13011; Q8WVX0; Q96EZ9; A8K745
• Molecular Weight: 35,816 Da
• NCBI Official Full Name: delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial
• NCBI Official Synonym Full Names: enoyl CoA hydratase 1, peroxisomal
• NCBI Official Symbol: ECH1
• NCBI Official Synonym Symbols: HPXEL
• NCBI Protein Information: delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial; delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial; delta3,5-delta2,4-dienoyl-CoA isomerase; enoyl Coenzyme A hydratase 1, peroxisomal; peroxisomal enoyl-CoA hydratase 1
• UniProt Protein Name: Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial
• UniProt Gene Name: ECH1
• UniProt Entry Name: ECH1_HUMAN

NCBI Description

This gene encodes a member of the hydratase/isomerase superfamily. The gene product shows high sequence similarity to enoyl-coenzyme A (CoA) hydratases of several species, particularly within a conserved domain characteristic of these proteins. The encoded protein, which contains a C-terminal peroxisomal targeting sequence, localizes to the peroxisome. The rat ortholog, which localizes to the matrix of both the peroxisome and mitochondria, can isomerize 3-trans,5-cis-dienoyl-CoA to 2-trans,4-trans-dienoyl-CoA, indicating that it is a delta3,5-delta2,4-dienoyl-CoA isomerase. This enzyme functions in the auxiliary step of the fatty acid beta-oxidation pathway. Expression of the rat gene is induced by peroxisome proliferators. [provided by RefSeq, Jul 2008]

Uniprot Description

ECH1: Isomerization of 3-trans,5-cis-dienoyl-CoA to 2-trans,4- trans-dienoyl-CoA. Belongs to the enoyl-CoA hydratase/isomerase family.

Protein type: Amino Acid Metabolism - tyrosine; Mitochondrial; Isomerase; EC 5.3.3.-

Chromosomal Location of Human Ortholog: 19q13.1

Cellular Component: membrane; mitochondrion; peroxisome

Molecular Function: protein binding; isomerase activity; receptor binding

Biological Process: fatty acid beta-oxidation