Rabbit anti-Human, Mouse EBP1 Polyclonal Antibody | anti-PA2G4 antibody

Anti-EBP1 Antibody, Rabbit Polyclonal

Cat. Num. AAA8101326

• Gene Names: PA2G4; EBP1; HG4-1; p38-2G4
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
• Purity: Protein A & Antigen Affinity
• Synonyms: EBP1; Polyclonal Antibody; Anti-EBP1 Antibody; Rabbit Polyclonal; EBP1/PA2G4 Antibody; Rabbit PAb; Antigen Affinity Purified; proliferation-associated 2G4; Anti-HG4-1 Antibody; Anti-p38-2G4 Antibody; anti-PA2G4 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human EBP1
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-PA2G4 antibody: Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:2000; IHC-P: 1:2500-1:10,000; ICC/IF: 1:1500-1:50,000; IP: 1-2uL/mg of lysate
• Immunogen: A synthetic peptide corresponding to the center region of the Human EBP1/PA2G4
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human EBP1/PA2G4, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-PA2G4 rabbit polyclonal antibody at 1:500 dilution Lane A: Jurkat Whole Cell Lysate Lane B: A431 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit  IgG H&L (Dylight 800)  at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:44 kDa Observed band size:46 kDa (We are unsure as to the identity of these extra bands.))

Immunohistochemistry (IHC)

(Immunochemical staining PA2G4 in human kidney with rabbit polyclonal antibody at 1:5000 dilution, formalin-fixed paraffin embedded sections.)

Immunohistochemistry (IHC)

(Immunochemical staining PA2G4 in human stomach with rabbit polyclonal antibody at 1:5000 dilution, formalin-fixed paraffin embedded sections.)

Immunofluorescence (IF)

(Immunofluorescence staining of PA2G4 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human PA2G4 polyclonal antibody (1:5000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green).Positive staining was localized to cytoplasm and nucleus.)

Immunoprecipitation (IP)

(MYOD1 was immunoprecipitated using: Lane A:0.5 mg A431 Whole Cell Lysate 1 uL anti-MYOD1 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-MYOD1 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:500 dilution Developed using the DAB staining technique. Performed under reducing conditions. Predicted band size: 35 kDa Observed band size: 45 kDa)

Immunohistochemistry (IHC)

(Immunochemical staining PA2G4 in mouse stomach with rabbit polyclonal antibody at 1:5000 dilution, formalin-fixed paraffin embedded sections.)

Immunohistochemistry (IHC)

(Immunochemical staining PA2G4 in mouse brain with rabbit polyclonal antibody at 1:5000 dilution, formalin-fixed paraffin embedded sections.)

Related Product Information for anti-PA2G4 antibody

EBP1, also known as PA2G4, is an RNA-binding protein that belongs to the peptidase M24 family. It can be detected n several cell lines tested, including primary and transformed cell lines. EBP1 also present in pre-ribosomal ribonucleoprotein complexes and may be involved in ribosome assembly and the regulation of intermediate and late steps of rRNA processing. This protein is a transcriptional co-repressor of androgen receptor-regulated genes and other cell cycle regulatory genes through its interactions with histone deacetylases. PA2G4 can interact with the cytoplasmic domain of the ErbB3 receptor and may contribute to transducing growth regulatory signals. EBP1 has been implicated in growth inhibition and the induction of differentiation of human cancer cells. It seems to be involved in growth regulation. EBP1 also mediates cap-independent translation of specific viral IRESs (internal ribosomal entry site).

References

Yoo J Y, et al. (2000) Interaction of the PA2G4 (EBP1) protein with ErbB-3 and regulation of this binding by heregulin. Br J Cancer. 82(3):683-90.Andersen JS, et al. (2002) Directed proteomic analysis of the human nucleolus. Curr Biol. 12(1):1-11.Lehner B, et al. (2004) A protein interaction framework for human mRNA degradation. Genome Res. 14 (7):1315-23.

NCBI and Uniprot Product Information

• NCBI GI #: 124494254
• NCBI GeneID: 5036
• NCBI Accession #: NP_006182.2
• NCBI GenBank Nucleotide #: NM_006191.2
• UniProt Accession #: Q9UQ80; O43846; Q9UM59
• Molecular Weight: 43,787 Da
• NCBI Official Full Name: proliferation-associated protein 2G4
• NCBI Official Synonym Full Names: proliferation-associated 2G4, 38kDa
• NCBI Official Symbol: PA2G4
• NCBI Official Synonym Symbols: EBP1; HG4-1; p38-2G4
• NCBI Protein Information: proliferation-associated protein 2G4; erbB3-binding protein 1; ErbB-3 binding protein 1; ErbB3-binding protein Ebp1; cell cycle protein p38-2G4 homolog
• UniProt Protein Name: Proliferation-associated protein 2G4
• Protein Family: Proliferation-associated protein
• UniProt Gene Name: PA2G4
• UniProt Synonym Gene Names: EBP1; hG4-1
• UniProt Entry Name: PA2G4_HUMAN

NCBI Description

This gene encodes an RNA-binding protein that is involved in growth regulation. This protein is present in pre-ribosomal ribonucleoprotein complexes and may be involved in ribosome assembly and the regulation of intermediate and late steps of rRNA processing. This protein can interact with the cytoplasmic domain of the ErbB3 receptor and may contribute to transducing growth regulatory signals. This protein is also a transcriptional co-repressor of androgen receptor-regulated genes and other cell cycle regulatory genes through its interactions with histone deacetylases. This protein has been implicated in growth inhibition and the induction of differentiation of human cancer cells. Six pseudogenes, located on chromosomes 3, 6, 9, 18, 20 and X, have been identified. [provided by RefSeq, Jul 2008]

Uniprot Description

Ebp1: May play a role in a ERBB3-regulated signal transduction pathway. Seems be involved in growth regulation. Acts a corepressor of the androgen receptor (AR) and is regulated by the ERBB3 ligand neuregulin-1/heregulin (HRG). Inhibits transcription of some E2F1-regulated promoters, probably by recruiting histone acetylase (HAT) activity. Binds RNA. Associates with 28S, 18S and 5.8S mature rRNAs, several rRNA precursors and probably U3 small nucleolar RNA. May be involved in regulation of intermediate and late steps of rRNA processing. May be involved in ribosome assembly. Mediates cap-independent translation of specific viral IRESs (internal ribosomal entry site). Belongs to the peptidase M24 family.

Protein type: Nucleolus; Transcription, coactivator/corepressor; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 12q13.2

Cellular Component: nucleoplasm; membrane; cytoplasm; nucleolus; nucleus; ribonucleoprotein complex

Molecular Function: protein binding; DNA binding; ubiquitin protein ligase binding; transcription factor activity

Biological Process: regulation of translation; cell proliferation; transcription, DNA-dependent; positive regulation of cell differentiation; negative regulation of transcription, DNA-dependent; cell cycle arrest; rRNA processing; negative regulation of apoptosis