Rabbit anti-Human Early B-Cell Factor 1 Polyclonal Antibody | anti-EBF1 antibody

Polyclonal Antibody to Early B-Cell Factor 1 (EBF1)

Cat. Num. AAA2027769

• Gene Names: EBF1; EBF; COE1; OLF1; O/E-1
• Reactivity: Human
• Applications: Western Blot, Immunocytochemistry, Immunohistochemistry, ELISA, Immunoprecipitation, Immunofluorescence, Flow Cytometry
• Purity: Antigen-specific Affinity Chromatography.
• Synonyms: Early B-Cell Factor 1; Polyclonal Antibody; Polyclonal Antibody to Early B-Cell Factor 1 (EBF1); anti-EBF1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: The antibody is a rabbit polyclonal antibody raised against EBF1. It has beenselected for its ability to recognize EBF1 in immunohistochemical staining andwestern blotting.
• Purity/Purification: Antigen-specific Affinity Chromatography.
• Form/Format: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
• Concentration: 500ug/mL (varies by lot)
• Sequence: Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGS- DP VIIDRFFLKF FLKCNQNCLK NAGNPRDMRR FQVVVSTTVN VDGHVLAVSD NMFVHNNSKH GRRARRLDPS EGTPSYLEHA TPCIKAISPS EGWTTGGATV IIIGDNFFDG LQVIFGTMLV WSELITPHAI RVQTPPRHIP GVVEVTLSYK SKQFCKGTPG RFIYTALNEP TIDYGFQRLQ KVIPRHPGDP ERLPKEVILK RAADLVEALY GMPHNNQEII LKRAADIAEA LYSVPRNHNQ LPALANTSVH

• Applicable Applications for anti-EBF1 antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA), IP, IF, FCM.
• Application Notes: Western blotting: 0.5-2ug/ml; Immunocytochemistry in formalin fixed cells: 5-20ug/ml; Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml; Immunohistochemistry in paraffin section: 5-20ug/ml; Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml; Optimal working dilutions must be determined by end user.
• Fragment: EBF1 (Asp179~Ser451)
• Organism Species: Homo sapiens (Human)
• Source: Polyclonal antibody preparation
• Cross Reactivity: Human, Rat
• Conjugate: No Conjugate
• Immunogen: Recombinant Early B-Cell Factor 1 (EBF1)
• Traits: Liquid
• Conjugated Antibody: The APC conjugated antibody version of this item is also available as catalog #MBS2055011
• Preparation and Storage: Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.; Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed.The loss rate is less than 5% within the expiration date under appropriate storage condition.

Western Blot (WB)

(Western Blot: Sample: Mouse Sp2/0 cell lysate; Primary Ab: 3ug/ml Rabbit Anti-Human EBF1 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (#MBS2086047))

Western Blot (WB)

(Western Blot: Sample: Recombinant protein.)

Immunohistochemistry (IHC)

(DAB staining on fromalin fixed paraffin-embedded Heart tissue))

Knockout Validation

(Knockout Validation: Lane 1: Wild-type Raji cell lysate; Lane 2: EBF1 knockout Raji cell lysate; Predicted MW: 64kDa Observed MW: 60kDa Primary Ab: 3ug/ml Rabbit Anti-Human EBF1 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (#MBS2086047))

Product Categories/Family for anti-EBF1 antibody

Knock-Out Validation Antibody

NCBI and Uniprot Product Information

• NCBI GI #: 31415878
• NCBI GeneID: 1879
• NCBI Accession #: NP_076870.1
• NCBI GenBank Nucleotide #: NM_024007.4
• UniProt Accession #: Q9UH73; Q8IW11
• Molecular Weight: 61,059 Da
• NCBI Official Full Name: transcription factor COE1 isoform 2
• NCBI Official Synonym Full Names: early B cell factor 1
• NCBI Official Symbol: EBF1
• NCBI Official Synonym Symbols: EBF; COE1; OLF1; O/E-1
• NCBI Protein Information: transcription factor COE1
• UniProt Protein Name: Transcription factor COE1
• Protein Family: EIN3-binding F-box protein
• UniProt Gene Name: EBF1
• UniProt Synonym Gene Names: COE1; EBF; O/E-1; OE-1

Uniprot Description

EBF1: Transcriptional activator which recognizes variations of the palindromic sequence 5'-ATTCCCNNGGGAATT-3'. Belongs to the COE family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription regulation

Chromosomal Location of Human Ortholog: 5q33.3

Cellular Component: nucleus

Molecular Function: DNA binding; metal ion binding; protein binding

Biological Process: multicellular organism development; regulation of transcription, DNA-templated; transcription, DNA-dependent