Rabbit E2F4 Polyclonal Antibody | anti-E2F4 antibody

Anti-E2F4 Antibody, Rabbit Polyclonal

Cat. Num. AAA8100780

• Gene Names: E2F4; E2F-4
• Reactivity: Human; Predicted to React with: Mouse (Species predicted to react based on 100% sequence homology)
• Applications: Western Blot, Immunocytochemistry, Immunofluorescence
• Purity: Protein A & Antigen Affinity
• Synonyms: E2F4; Polyclonal Antibody; Anti-E2F4 Antibody; Rabbit Polyclonal; E2F4 Antibody; Rabbit PAb; Antigen Affinity Purified; Anti-E2F-4 Antibody; anti-E2F4 antibody

• Host: Rabbit
• Reactivity: Human; Predicted to React with: Mouse (Species predicted to react based on 100% sequence homology)
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human E2F4
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; PBS, pH7.0 with 0.03% Proclin300

• Applicable Applications for anti-E2F4 antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:2000; ICC/IF: 1:100-1:500
• Immunogen: A synthetic peptide corresponding to the center region of the Human E2F4
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human E2F4, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-E2F4 rabbit polyclonal antibody at 1:500 dilutionLane A: K562 Whole Cell LysateLane B: Raji Whole Cell LysateLane C: HeLa Whole Cell LysateLane D: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:44 kDaObserved band size:53 kDa)

Immunofluorescence (IF)

(Immunofluorescence staining of E2F4 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human E2F4 polyclonal antibody (dilution ratio 1:200) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.)

Related Product Information for anti-E2F4 antibody

E2F4 is an important basal transcription factor with the potential to promote tumor growth. The transcription factor E2F4 controls the proliferation of normal and cancerous intestinal epithelial cells. E2F4 localization in normal human intestinal epithelial cells (HIEC) is cell cycle-dependent, being cytoplasmic in quiescent differentiated cells but nuclear in proliferative cells. Contrary to other E2Fs, the coding region of the E2F4 gene contains a longer spacer segment of a CAG trinucleotide repeat sequence encoding 13 consecutive serine residues, which is highly vulnerable to frameshift mutations in situations of genetic instability. Cancer-associated E2F4 mutations enhance the capacity of colorectal cancer cells to grow without anchorage, thereby contributing to tumor progression. E2F4 is a transcriptional repressor implicated in cell cycle arrest and whose repressive activity depends on its interaction with members of the RB family.

Product Categories/Family for anti-E2F4 antibody

Apoptosis Transcription Factors and Regulators

References

Paquin MC, et al. (2013) Functional impact of colorectal cancer-associated mutations in the transcription factor e2f4. Int J Oncol 43 (6): 2015-2022.Paquin MC, et al. (2013) Erk-associated changes in e2f4 phosphorylation, localization and transcriptional activity during mitogenic stimulation in human intestinal epithelial crypt cells. BMC Cell Biol 14 33.Hsu J, et al. (2016) Novel functions for the transcription factor e2f4 in development and disease. Cell Cycle 15 (23): 3183-3190.Farman FU, et al. (2018) Aberrant promoter methylation status is associated with upregulation of the e2f4 gene in breast cancer. Oncol Lett 15 (6): 8461-8469.

NCBI and Uniprot Product Information

• NCBI GI #: 2494229
• NCBI GeneID: 1874
• UniProt Accession #: Q16254; Q12991; Q15328; A6NGR8; B5BU56
• Molecular Weight: 43,960 Da
• NCBI Official Full Name: Transcription factor E2F4
• NCBI Official Synonym Full Names: E2F transcription factor 4, p107/p130-binding
• NCBI Official Symbol: E2F4
• NCBI Official Synonym Symbols: E2F-4
• NCBI Protein Information: transcription factor E2F4; p107/p130-binding protein
• UniProt Protein Name: Transcription factor E2F4
• Protein Family: Transcription factor
• UniProt Gene Name: E2F4
• UniProt Synonym Gene Names: E2F-4
• UniProt Entry Name: E2F4_HUMAN

NCBI Description

The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein binds to all three of the tumor suppressor proteins pRB, p107 and p130, but with higher affinity to the last two. It plays an important role in the suppression of proliferation-associated genes, and its gene mutation and increased expression may be associated with human cancer. [provided by RefSeq, Jul 2008]

Uniprot Description

E2F4: Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC- 3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F4 binds with high affinity to RBL1 and RBL2. In some instances can also bind RB1. Belongs to the E2F/DP family.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 16q22.1

Cellular Component: nucleoplasm; transcription factor complex

Molecular Function: protein domain specific binding; protein binding; DNA binding; transcription factor activity; transcription factor binding

Biological Process: G1/S-specific transcription in mitotic cell cycle; transcription initiation from RNA polymerase II promoter; organ morphogenesis; transcription, DNA-dependent; blood circulation; transforming growth factor beta receptor signaling pathway; epithelial cell development; gene expression; positive regulation of transcription from RNA polymerase II promoter; mitotic cell cycle; cell volume homeostasis; regulation of cell size; regulation of cell proliferation