Rabbit E2F2 Polyclonal Antibody | anti-E2F2 antibody

Anti-E2F2 antibody

Cat. Num. AAA176024

• Gene Names: E2F2; E2F-2
• Reactivity: Reacts with: Human, rat; Predicted to work with: Mouse
• Applications: Western Blot
• Purity: Immunogen affinity purified.
• Synonyms: E2F2; Polyclonal Antibody; Anti-E2F2 antibody; Transcription factor E2F2(E2F-2); E2F transcription factor 2; dE2F2 antibody; E2F transcription factor 2 antibody; E2F-2 antibody; E2F2 antibody; E2F2_HUMAN antibody; Transcription factor E2F2 antibody; anti-E2F2 antibody

• Host: Rabbit
• Reactivity: Reacts with: Human, rat; Predicted to work with: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: No cross reactivity with other proteins.
• Purity/Purification: Immunogen affinity purified.
• Form/Format: Lyophilized
• Sequence Length: 437

• Applicable Applications for anti-E2F2 antibody: Western Blot (WB)
• Application Notes: Western Blot (WB):; Concentration: 0.1-0.5ug/ml; Tested Species: Hu, Rat; Predicted Species: Ms; Tested Species: In-house tested species with positive results.; Predicted Species: Species predicted to be fit for the product based on sequence similarities.; Other applications have nto been tested.; Optimal dilutions shoudl be determined by end users.
• Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human E2F2 (422-427aa ISDLFDSYDLGDLLIN), identical to the related mouse sequence.
• Contents: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB)

(Anti-E2F2 antibody, MBS176024, Western blottingAll lanes: Anti E2F2 (MBS176024) at 0.5ug/mlLane 1: Rat Lung Tissue Lysate at 50ugLane 2: Rat Heart Tissue Lysate at 50ugLane 3: Rat Brain Tissue Lysate at 50ugLane 4: Rat Kidney Tissue Lysate at 50ugLane 5: HELA Whole Cell Lysate at 40ugLane 6: COLO320 Whole Cell Lysate at 40ugLane 7: A549 Whole Cell Lysate at 40ugLane 8: MCF-7 Whole Cell Lysate at 40ugLane 9: SMMC Whole Cell Lysate at 40ugPredicted bind size: 48KDObserved bind size: 48KD )

Related Product Information for anti-E2F2 antibody

Description: Rabbit IgG polyclonal antibody for Transcription factor E2F2(E2F2) detection. Tested with WB in Human, Mouse, Rat.
Background: E2F2(E2F transcription factor 2) also called E2F-2, is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F2 gene is mapped to 1p36 by fluorescence in situ hybridization. Electrophoretic mobility shift assays revealed the specific binding of E2F2 to ECE1b promoter sequences containing either allele of the C-338A polymorphism, with the -338A allele being associated with an increased affinity to E2F2 compared with -338C. The ability of Myc to induce S phase was impaired in the absence of either E2f2 or E2f3 but not E2f1 or E2f4. In contrast, the ability of Myc to induce apoptosis was markedly reduced in cells deleted for E2f1 but not E2f2 or E2f3.

NCBI and Uniprot Product Information

• NCBI GI #: 2494228
• NCBI GeneID: 1870
• UniProt Accession #: Q14209; Q7Z6H1; B2R9W1
• Molecular Weight: 47,506 Da
• NCBI Official Full Name: Transcription factor E2F2
• NCBI Official Synonym Full Names: E2F transcription factor 2
• NCBI Official Symbol: E2F2
• NCBI Official Synonym Symbols: E2F-2
• NCBI Protein Information: transcription factor E2F2
• UniProt Protein Name: Transcription factor E2F2
• Protein Family: Transcription factor
• UniProt Gene Name: E2F2
• UniProt Synonym Gene Names: E2F-2
• UniProt Entry Name: E2F2_HUMAN

NCBI Description

The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F1 and E2F3, have an additional cyclin binding domain. This protein binds specifically to retinoblastoma protein pRB in a cell-cycle dependent manner, and it exhibits overall 46% amino acid identity to E2F1. [provided by RefSeq, Jul 2008]

Uniprot Description

E2F2: Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC- 3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from g1 to s phase. E2F2 binds specifically to RB1 in a cell-cycle dependent manner. Belongs to the E2F/DP family.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 1p36

Cellular Component: nucleoplasm; transcription factor complex

Molecular Function: protein binding; DNA binding; transcription factor binding; transcription factor activity

Biological Process: transcription initiation from RNA polymerase II promoter; regulation of transcription, DNA-dependent; regulation of cell cycle; mitotic cell cycle